A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification
A universal constructing method for high performance DNA biosensors based on the optimized photoelectrode material and dual recycling amplification
复制标题
基于优化光电极材料和双循环放大的高性能DNA生物传感器通用构建方法
DOI：
10.1016/j.apsusc.2022.152661
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发表时间：
2022
影响因子：
6.7
通讯作者：
Xiao Dan
中科院分区：
文献类型：
--
作者：
Chen Delun;Dong Fan;Zou Xue;Qiao Bin;Wang Xiaohong;Zhang Kexi;Tu Jinchun;Zhen Chao;Wu Qiang;Xiao Dan
过去几十年来，人们开发了许多有效的光电化学 DNA 传感器策略。他们大多关注检测性能，包括灵敏度、检测限和线性范围，但很少关注光电极材料的关键作用和策略的通用性。在此，首次利用密度泛函理论对光电极材料进行优化，以提高DNA生物传感器的综合性能。并讨论了基于不同核酸序列的双循环扩增的通用策略。经过对检测条件的系统优化，I27L基因DNA序列的检测实现了10-16至10-6M的大线性范围、5.66 μA·cm−2·dec-1的高灵敏度和10-18M（每微升基因拷贝数）的低检测限。最后，通过对恙虫病东方体DNA序列的检测，证明了该方法的普适性。
Many effective strategies for photoelectrochemical DNA sensors have been developed in the past decades. Most of them focused on the detection performance including sensitivity, limit of detection and linear range, but few on the pivotal roles of photoelectrode materials and the universality for strategies. Herein, the photoelectrode material was optimized by density functional theory for improving the comprehensive performances of DNA biosensor for the first time. And the universal strategies, based on dual recycling amplification, was discussed by different nucleic acid sequences. After systematical optimization of detection conditions, a large linear range of 10-16to 10-6M, high sensitivity of 5.66 μA·cm−2·dec-1and low limit of detection of 10-18M (a gene copy per microliter) were achieved for the detection of DNA sequences from the I27L gene. Finally, detection of DNA sequences fromOrientia tsutsugamushiwas used to prove the universality of the novel method.