Asymmetry of Early Endosome Distribution in C. elegans Embryos
Asymmetry of Early Endosome Distribution in C. elegans Embryos
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DOI：
10.1371/journal.pone.0000493
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发表时间：
2007-06-06
期刊：
影响因子：
3.7
通讯作者：
Ahringer, Julie
中科院分区：
文献类型：
--
作者：
Andrews, Robert;Ahringer, Julie
背景。内吞作用参与许多细胞事件的调节，包括信号传导、细胞迁移和细胞极性。为了开始研究内吞作用在早期秀丽隐杆线虫发育中的作用，我们检查了胚胎中早期内体（EE）的分布和动态。方法/主要发现。 EE 主要存在于细胞外围，受精后最初分布均匀。引人注目的是，我们发现在第一个细胞周期中，EEA-1 阳性 EE 在前皮层变得丰富。相比之下，高尔基室在分布上没有表现出不对称性。 EE 的不对称富集取决于肌动球蛋白收缩性和胚胎 PAR 极性。除了位于皮层之外，EE 还存在于中心体周围。这些 EE 快速（1.3um/s）从皮层直接移动到中心体，其速度与负端定向运动动力蛋白的速度相当。结论/意义。我们推测早期内体的不对称性可能在细胞不对称性或命运决定中发挥作用。
Background. Endocytosis is involved in the regulation of many cellular events, including signalling, cell migration, and cell polarity. To begin to investigate roles for endocytosis in early C. elegans development, we examined the distribution and dynamics of early endosomes (EEs) in embryos. Methodology/Principal Findings. EEs are primarily found at the cell periphery with an initially uniform distribution after fertilization. Strikingly, we find that during the first cell cycle, EEA-1 positive EEs become enriched at the anterior cortex. In contrast, the Golgi compartment shows no asymmetry in distribution. Asymmetric enrichment of EEs depends on acto-myosin contractility and embryonic PAR polarity. In addition to their localization at the cortex, EEs are also found around the centrosome. These EEs move rapidly (1.3um/s) from the cortex directly to the centrosome, a speed comparable to that of the minus end directed motor dynein. Conclusions/Significance. We speculate that the asymmetry of early endosomes might play a role in cell asymmetries or fate decisions.