Differences in apoptotic susceptibility of cytotrophoblasts and syncytiotrophoblasts in normal pregnancy to those complicated with preeclampsia and intrauterine growth restriction
Differences in apoptotic susceptibility of cytotrophoblasts and syncytiotrophoblasts in normal pregnancy to those complicated with preeclampsia and intrauterine growth restriction
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DOI：
10.1016/s0002-9440(10)63857-6
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发表时间：
2003-02-01
影响因子：
6
通讯作者：
Baker, PN
中科院分区：
文献类型：
--
作者：
Crocker, IP;Cooper, S;Baker, PN
胎盘凋亡在子痫前期（PE）和宫内生长受限（IUGR）患者体内增加。这种现象的原因和病理意义尚不清楚。本研究考虑了正常妊娠、PE妊娠和IUGR妊娠的绒毛滋养细胞的凋亡易感性。采用体外培养的细胞滋养细胞（CTs）和体外合胞模型。从12例正常妊娠、12例PE妊娠和12例IUGR妊娠足月胎盘中分离ct。凋亡通过末端dUTP镍端标记（TUNEL）、膜联蛋白V结合和ADP:ATP比值测定。用肿瘤坏死因子/干扰素或还原氧刺激细胞(
Placental apoptosis is increased in vivo in preeclampsia (PE) and intrauterine growth restriction (IUGR). The cause and pathological implications of this phenomenon are unknown. This study considers the apoptotic susceptibility of villous trophoblasts from normal, PE, and IUGR pregnancies. Cultured cytotro-phoblasts (CTs) and an in vitro model of syncytialization were used. CTs were isolated from term placentas of 12 normal, 12 PE, and 12 IUGR pregnancies. Apoptosis was determined by terminal dUTP nick-end labeling (TUNEL), Annexin V binding, and ADP:ATP ratios. Cells were stimulated with tumor necrosis factor-alpha/interferon-gamma or reduced oxygen (