Oral collagen-derived dipeptides, prolyl-hydroxyproline and hydroxyprolyl-glycine, ameliorate skin barrier dysfunction and alter gene expression profiles in the skin
Oral collagen-derived dipeptides, prolyl-hydroxyproline and hydroxyprolyl-glycine, ameliorate skin barrier dysfunction and alter gene expression profiles in the skin
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DOI：
10.1016/j.bbrc.2014.12.006
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发表时间：
2015-01-09
影响因子：
3.1
通讯作者：
Mano, Hiroshi
中科院分区：
文献类型：
--
作者：
Shimizu, Jun;Asami, Naoto;Mano, Hiroshi
口服补充胶原水解物（CH）已被证明可以改善人类和实验动物的皮肤状况。先前在摄入CH后的人外周血中检测到几种含羟脯氨酸的寡肽，并且已经提出两种二肽，脯氨酰-羟脯氨酸（PO）和羟脯氨酰-甘氨酸（OG）对人体健康具有有益作用。当HR-1无毛小鼠喂食缺乏镁和锌的HR-AD饮食时，经表皮水分丢失（TEWL）增加，角质层含水量降低。在本研究中，我们研究了饮食PO和OG对HR-1无毛小鼠皮肤屏障功能障碍的影响。连续35天给小鼠喂食含或不含PO（0.15%）和OG（0.15%）的HR-AD饮食。PO和OG的给药显著降低TEWL，并显著增加角质层含水量。背侧皮肤的DNA微阵列分析揭示了PO和OG给药组与对照组之间的基因表达差异。我们还确定了肌肉相关的基因本体作为分析上调基因的结果。这些结果表明，PO和OG的管理改善皮肤屏障功能障碍和改变肌肉相关基因的表达。(C)2014 Elsevier Inc. All rights reserved.
Oral supplementation with collagen hydrolysate (CH) has been shown to improve the condition of the skin in humans and experimental animals. Several hydroxyproline-containing oligo-peptides were previously detected in human peripheral blood after the ingestion of CH, and the two dipeptides, prolyl-hydroxyproline (PO) and hydroxyprolyl-glycine (OG), have been proposed to have beneficial effects on human health. When HR-1 hairless mice were fed a HR-AD diet, which lacked magnesium and zinc, transepidermal water loss (TEWL) increased and water content of stratum corneum decreased. In the present study, we investigated the effects of dietary PO and OG on skin barrier dysfunction in HR-1 hairless mice. Mice were fed a HR-AD diet with or without PO (0.15%) and OG (0.15%) for 35 consecutive days. The administration of PO and OG significantly decreased TEWL, and significantly increased water content of stratum corneum. A DNA microarray analysis of the dorsal skin revealed differences in gene expression between the group administered PO and OG and the control group. We also identified muscle-related Gene Ontology as a result of analyzing the up-regulated genes. These results suggested that the administration of PO and OG improved skin barrier dysfunction and altered muscle-related gene expression. (C) 2014 Elsevier Inc. All rights reserved.