Evolutionary history of the ancient cutinase family in five filamentous Ascomycetes reveals differential gene duplications and losses and in Magnaporthe grisea shows evidence of sub- and neo-functionalization.
Evolutionary history of the ancient cutinase family in five filamentous Ascomycetes reveals differential gene duplications and losses and in Magnaporthe grisea shows evidence of sub- and neo-functionalization.
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DOI：
10.1111/j.1469-8137.2008.02598.x
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发表时间：
2008-11
期刊：
影响因子：
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通讯作者：
Pari Skamnioti;R. F. Furlong;S. Gurr
中科院分区：
文献类型：
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作者：
Pari Skamnioti;R. F. Furlong;S. Gurr
*角质层是系统性或机会性地寄生植物的真菌的第一道屏障。本文报道了植物病原性子囊菌Magnaporthe grisea、禾谷镰刀菌Fusarium graminearum和灰霉菌Botrytis cinerea以及腐生性子囊菌Aspergillusnidulans和粗大脉孢霉(Neuropora Crassa)的多成员角质酶家族的进化历史。*所有真菌角质酶的分子分类表明，明确划分为两个古老的亚家族。没有发现从原核生物进行横向基因转移的证据。5个子囊菌的角质酶具有显著的拷贝数差异，它们形成6个分支，它们的极端序列多样性因缺乏一致的内含子而突出。基因复制与丢失的平均比例为2：3，但稻瘟病菌和粗粒稻瘟病菌分别表现出极端的家族扩张和收缩。*体内详细的转录图谱，将稻瘟病菌角质酶分为四种调控模式。系统发育相关角质酶基因的对称或不对称表达谱分别提示亚功能化和新功能化。*讨论了每种真菌的角质酶家族大小与基因组特征和生活方式的关系。角质酶基因家族的祖先，以及其个体成员的表达差异，为我们提供了对真菌生态位分化驱动因素的第一次洞察。
* The cuticle is the first barrier for fungi that parasitize plants systematically or opportunistically. Here, the evolutionary history is reported of the multimembered cutinase families of the plant pathogenic Ascomycetes Magnaporthe grisea, Fusarium graminearum and Botrytis cinerea and the saprotrophic Ascomycetes Aspergillus nidulans and Neurospora crassa. * Molecular taxonomy of all fungal cutinases demonstrates a clear division into two ancient subfamilies. No evidence was found for lateral gene transfer from prokaryotes. The cutinases in the five Ascomycetes show significant copy number variation, they form six clades and their extreme sequence diversity is highlighted by the lack of consensus intron. The average ratio of gene duplication to loss is 2 : 3, with the exception of M. grisea and N. crassa, which exhibit extreme family expansion and contraction, respectively. * Detailed transcript profiling in vivo, categorizes the M. grisea cutinases into four regulatory patterns. Symmetric or asymmetric expression profiles of phylogenetically related cutinase genes suggest subfunctionalization and neofunctionalization, respectively. * The cutinase family-size per fungal species is discussed in relation to genome characteristics and lifestyle. The ancestry of the cutinase gene family, together with the expression divergence of its individual members provides a first insight into the drivers for niche differentiation in fungi.