Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy.
Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy.
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DOI：
10.1039/c7sc01837a
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发表时间：
2017-08-01
期刊：
影响因子：
8.4
通讯作者：
Hulme AN
中科院分区：
文献类型：
--
作者：
Tipping WJ;Lee M;Serrels A;Brunton VG;Hulme AN
受激拉曼散射（SRS）显微镜与生物正交拉曼标记串联可以在活细胞中测量细胞内药物浓度，分布和治疗反应。受激拉曼散射（SRS）显微镜与生物正交拉曼标记策略串联，旨在彻底改变细胞内药物摄取的直接可视化。对一系列拉曼活性标签的合理评价使得鉴定出对母体药物生物功效干扰最小的高活性标签成为可能。药物摄取与细胞组成和细胞周期状态的标志物相关，并通过双色和多模态成像绘制细胞内结构。与SRS显微镜相关的最小光毒性和低光漂白使活细胞实时成像成为可能。这些研究证明了SRS显微镜在药物开发过程中的潜力。
Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling enables intracellular drug concentrations, distribution and therapeutic response to be measured in living cells. Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.
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DOI：
10.1073/pnas.0608156103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Chen, Shuibing;Do, Jeong Tae;Ding, Sheng
通讯作者：
Ding, Sheng
影响因子：
5.6
作者：
Dawson JC;Carragher NO
通讯作者：
Carragher NO
影响因子：
4.4
作者：
Lee, JY;Suh, SB;Kim, KS
通讯作者：
Kim, KS
影响因子：
3.7
作者：
Bogdanov AM;Kudryavtseva EI;Lukyanov KA
通讯作者：
Lukyanov KA
影响因子：
15
作者：
Fu, Dan;Yu, Yong;Folick, Andrew;Currie, Erin;Farese, Robert V., Jr.;Tsai, Tsung-Huang;Xie, Xiaoliang Sunney;Wang, Meng C.
通讯作者：
Wang, Meng C.