Compact microfluidic device for rapid concentration of PET tracers
Compact microfluidic device for rapid concentration of PET tracers
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DOI：
10.1039/c4lc00286e
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发表时间：
2014-01-01
期刊：
影响因子：
6.1
通讯作者：
van Dam, R. Michael
中科院分区：
文献类型：
--
作者：
Tseng, Wei-Yu;van Dam, R. Michael
正电子发射断层扫描（PET）示踪剂的HPLC纯化和重新配制可能导致最终产品的显著稀释，使得难以产生足够高的放射性浓度用于某些应用（例如小动物成像，体外测定和用辅基标记蛋白质）。这对于具有冗长或低产率合成的分子尤其如此。以更多的放射性开始合成增加了最终的放射性浓度，但增加了危险和处理的复杂性。另一种方法是在下游使用之前通过旋转蒸发等工艺浓缩最终产品。由于旋转蒸发器需要在热室中占用大量空间，以便更有效地使用，因此我们开发了一种紧凑的微流体系统，用于PET示踪剂的浓缩。该系统在可重复性、接口和自动化潜力方面也具有优势。我们在这里提出的系统的设计和性能表征，并证明在水基HPLC移动的相的几种示踪剂的浓度。
HPLC purification and reformulation of positron emission tomography (PET) tracers can lead to significant dilution of the final product, making it difficult to produce a sufficiently high radioactivity concentration for some applications (e.g. small animal imaging, in vitro assays, and labelling of proteins with prosthetic groups). This is especially true for molecules with lengthy or low-yield syntheses. Starting the synthesis with more radioactivity increases the final radioactivity concentration but increases hazards and complexity of handling. An alternative is to concentrate the final product by a process such as rotary evaporation prior to downstream use. Because a rotovap requires significant space within a hot cell that could be put to more productive use, we developed a compact microfluidic system for concentration of PET tracers. This system also provides advantages in terms of repeatability, interfacing and potential for automation. We present here the design and performance characterization of the system, and demonstrate the concentration of several tracers in aqueous-based HPLC mobile phases.