Creation of High-Yield Polyhydroxyalkanoates Engineered Strains by Low Energy Ion Implantation
Creation of High-Yield Polyhydroxyalkanoates Engineered Strains by Low Energy Ion Implantation
复制标题
通过低能离子注入创建高产聚羟基脂肪酸酯工程菌株
DOI：
10.1088/1009-0630/10/6/23
复制
发表时间：
2008
影响因子：
1.7
通讯作者：
Cheng Ying
中科院分区：
文献类型：
--
作者：
Qian Shiquan;Zhu Suwen;Cheng Beijiu;Cheng Ying
聚羟基脂肪酸酯（PHAs）是一种可生物降解的塑料材料，可以由多种微生物合成。然而，由于其生产成本与化学合成塑料相比较高，因此许多研究都集中在使用不同方法有效生产PHA上。本文研究了低能离子注入pCB 4菌株对PHA产生的诱变效应。结果表明，在10 keV、7.8 × 1014 N+/cm ~ 2的离子注入条件下，经多次诱变，获得了一株遗传稳定性较高的高产PHA菌株。优化发酵条件后，pCBH 4的生物量、PHAs浓度和PHAs含量分别达到2.26 g/L、1.81 g/L和80.08%，而野生型对照的生物量、PHAs浓度和PHAs含量分别为1.24 g/L、0.61 g/L和49.20%。此外，在突变菌株中，PHA的主要成分被鉴定为聚-3-羟基丁酸酯（PHB），该化合物的产量提高到41.33%，而野生型菌株的产量为27.78%。
Polyhydroxyalkanoates (PHAs), as a candidate for biodegradable plastic materials, can be synthesized by numerous microorganisms. However, as its production cost is high in comparison with those of chemically synthesized plastics, a lot of research has been focused on the efficient production of PHAs using different methods. In the present study, the mutation effects of PHAs production in strain pCB4 were investigated with implantation of low energy ions. It was found that under the implantation conditions of 7.8 × 1014 N+/cm2 at 10 keV, a high-yield PHAs strain with high genetic stability was generated from many mutants. After optimizing its fermentation conditions, the biomass, PHAs concentration and PHAs content of pCBH4 reached 2.26 g/L, 1.81 g/L, and 80.08% respectively, whereas its wild type controls were about 1.24 g/L, 0.61 g/L, and 49.20%. Moreover, the main constituent of PHAs was identified as poly-3-hydroxybutyrates (PHB) in the mutant stain and the yield of this compound was increased up to 41.33% in contrast to that of 27.78% in the wild type strain.
DOI：
10.1007/b135662
发表时间：
2005-10
期刊：
--
影响因子：
--
作者：
Zengliang Yu;Liangdeng Yu;T. Vilaithong;I. Brown
通讯作者：
Zengliang Yu;Liangdeng Yu;T. Vilaithong;I. Brown