Impact of Tetracycline Antibiotic on the Transcriptional Expression of Tetracycline Resistance Genes in Shigella flexneri
Impact of Tetracycline Antibiotic on the Transcriptional Expression of Tetracycline Resistance Genes in Shigella flexneri
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DOI：
10.13227/j.hjkx.202001081
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发表时间：
2020-08-15
期刊：
影响因子：
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通讯作者：
Qian Ya-jie
中科院分区：
文献类型：
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作者：
Gao Pin;Ruan Xiao-hui;Qian Ya-jie
四环素类抗生素广泛存在于不同的环境基质中。从活性污泥中筛选纯化福氏志贺菌的四环素抗性菌（TRB），采用定量PCR和反转录PCR方法研究TC胁迫对TRB中tetC、tetO和tetX基因丰度和表达水平的影响。讨论了TC浓度与TC-ARGs基因丰度及其表达水平的相关性。结果表明，TC胁迫对福氏志贺菌在整个培养周期（24 h）内的生长均有抑制作用，且随着TC浓度的增加，菌体浓度的生长速率降低。TC胁迫可促进福氏志贺菌TC-ARGs基因的表达，tetC、tetO和tetX基因的表达量呈现先升高后降低的趋势。相关性分析结果表明，TC浓度与TC-ARGs基因丰度及其表达水平之间无显著相关性。然而，tetC和tetO的基因丰度与其表达水平显著相关，因此表明它们可以在一定程度上用于评估和评估表达水平。
The tetracycline (TC) antibiotic has been widely found in different environmental matrices. The tetracycline resistant bacterium (TRB) of Shigella flexneri was screened and purified from activated sludge, and was then used to study the impact of TC stress on the gene abundances and expression levels of TC resistance genes (TC-ARGs), including tetC, tetO, and tetX, which were respectively quantified by quantitative PCR and reverse transcriptional PCR. Correlations between the TC concentration and gene abundances of TC-ARGs and their expression levels were discussed. The results showed that TC stress had an inhibiting effect on the growth of Shigella flexneri during the entire culture cycle (24 h) and that the growth rate of the bacterial concentration decreased with increasing TC concentration. However, less impact on the gene abundance of TC-ARGs was found. TC stress could promote the expression of TC-ARGs in Shigella flexneri, and the expression levels of tetC, tetO, and tetX genes first increased and then decreased. The correlation results indicated that no significant correlation was observed between the TC concentration and gene abundances of TC-ARGs and their expression levels. Nevertheless, the gene abundances of tetC and tetO were significantly correlated with their expression levels, thus indicating that they can be used to evaluate and assess expression levels to a certain extent.