SPONTANEOUS AND INDUCED MUTAGENESIS IN WESTERN EQUINE ENCEPHALOMYELITIS VIRUS IN CHICK-EMBRYO CELLS WITH DIFFERENT REPAIR ACTIVITY
SPONTANEOUS AND INDUCED MUTAGENESIS IN WESTERN EQUINE ENCEPHALOMYELITIS VIRUS IN CHICK-EMBRYO CELLS WITH DIFFERENT REPAIR ACTIVITY
复制标题
DOI：
10.1073/pnas.72.1.386
复制
发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
LVOVA, GN
中科院分区：
文献类型：
--
作者：
DUBININ, NP;ZASUKHINA, GD;LVOVA, GN
有证据表明，西方马脑脊髓炎病毒暴露于紫外线辐射和甲磺酸甲酯后，在商业和无白血病的鸡胚胎细胞中的存活率存在差异，其修复活性不同。当病毒在无白血病的鸡胚细胞中传代时，自发突变的水平（基于脑脊髓炎病毒小斑块变异的产量）并没有本质上改变，而在商业鸡胚细胞中观察到小斑块变异的数量增加。在使用甲基甲磺酸盐的商业鸡胚胎细胞实验中，与对照相比，诱导病毒变异的数量增加了10倍，而在无白血病细胞中没有发现病毒变异的诱导。
Evidence was obtained indicating differences in the survival rate of Western equine encephalomyelitis virus after exposure to ultraviolet radiation and methyl methanesulfonate in commercial and leukosisfree chick embryo cells that differed in repair activity. The levels of spontaneous mutagenesis (on the basis of the yield of small palque variants of the encephalomyelitis virus) did not essentially change when the virus was passage in leukosis-free chick embryo cells, whereas an increase in the number of small palque variants was observed in the cells of commercial chick embryos. A 10-fold increase in the number of induced virus variants was observed in commercial chick embryo cells in experiments with methyl methanesulfonate as compared with the contorl, whereas the induction of virus variants was not noted in leukosis-free cells.