Stability of cytoplasmic messenger RNA in HeLa cels.
Stability of cytoplasmic messenger RNA in HeLa cels.
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HeLa 细胞中细胞质信使 RNA 的稳定性。
DOI：
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发表时间：
1973
影响因子：
11.1
通讯作者：
G. Attardi
中科院分区：
文献类型：
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作者：
W. Murphy;G. Attardi
在HeLa细胞中测定了来自细胞质游离多聚核糖体的脉冲标记或长期标记信使RNA（mRNA）的代谢稳定性，追踪条件不涉及RNA合成抑制剂，并在苯甲酰化-DEAE-纤维素或聚（T）-纤维素上层析分离mRNA。对于这些研究，开发了一种新的追踪技术，该技术允许在短[（3）H]尿苷脉冲期间分析标记的mRNA的稳定性。脉冲标记和长期标记的mRNA被发现衰减，估计平均半衰期约为2和3天，分别比迄今为止假设的要长得多。
The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [(3)H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.