THIN-LAYER SPECTROELECTROCHEMICAL STUDY OF THE REVERSIBLE-REACTION BETWEEN NICOTINAMIDE ADENINE-DINUCLEOTIDE AND FLAVIN ADENINE-DINUCLEOTIDE
THIN-LAYER SPECTROELECTROCHEMICAL STUDY OF THE REVERSIBLE-REACTION BETWEEN NICOTINAMIDE ADENINE-DINUCLEOTIDE AND FLAVIN ADENINE-DINUCLEOTIDE
复制标题
DOI：
10.1016/0022-0728(91)85042-n
复制
发表时间：
1991-03-11
影响因子：
4.5
通讯作者：
COMTAT, M
中科院分区：
文献类型：
--
作者：
BERGEL, A;COMTAT, M
尽管热力学数据非常不利，但可以用还原形式的黄素腺嘌呤二核苷酸（FADH 2）来驱动NAD+的还原。 通过电化学薄层方法使平衡逆转，该方法能够达到高浓度的FADH 2。 只有在催化反应的假单胞菌的甲酸脱氢酶存在的情况下才发生还原。 测定了FAD的最大酶促反应速率和米氏常数。 相同的酶能够通过FAD催化NADH的氧化。 因此，可以设想在厌氧条件下，在低至-0.3 V（vs. SCE）的电位下间接安培检测NADH。 使用相同的化合物，简单地通过修改电极电位催化的双向反应的可能性，强调。
It is possible to drive the reduction of NAD+ with the reduced form of flavin adenine dinucleotide (FADH2) despite the very unfavourable thermodynamic data. The equilibrium is reversed by means of an electrochemical thin-layer method which enables a high concentration of FADH2 to be reached. The reduction occurs only in the presence of a formate dehydrogenase of Pseudomonas oxalaticus which catalyses the reaction. The maximum rate of enzymatic reaction and the Michaelis constant of FAD are evaluated. The same enzyme is able to catalyse the oxidation of NADH by FAD. The indirect amperometric detection of NADH at a potential as low as - 0.3 V (vs. SCE) can therefore be envisaged under anaerobic conditions. The possibility of catalysing the two-way reaction using the same compounds, simply by modifying the electrode potential, is emphasized.