HB-EGF expression as a potential biomarker of acquired middle ear cholesteatoma
HB-EGF expression as a potential biomarker of acquired middle ear cholesteatoma
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DOI：
10.1080/00016489.2017.1284343
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发表时间：
2017-01-01
影响因子：
1.4
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Xie, Shumin;Wang, Xiaoli;Liu, Wei
结论：肝素结合表皮生长因子样生长因子（HB-EGF）在胆脂瘤的发生发展和侵袭过程中发挥重要作用。本研究可能有助于认识胆脂瘤发病的分子机制，使HB-EGF成为胆脂瘤药物干预的一个有前景的靶点。目的：检测人获得性中耳胆脂瘤手术标本中HB-EGF的表达，分析其调节上皮角质形成细胞过度增殖的功能作用。方法：本研究共纳入34例接受中耳胆脂瘤手术治疗的患者。通过实时定量逆转录聚合酶链反应（RT-qPCR）、免疫组化染色和蛋白质印迹研究中耳胆脂瘤组织和正常耳后皮肤组织中HB-EGF mRNA和蛋白的表达。分析骨吸收程度与HB-EGF表达的相关性。 结果：平均而言，与正常耳后皮肤相比，RT-qPCR检测胆脂瘤上皮中HB-EGF mRNA表达量显着升高2.41倍，Western blot检测HB-EGF蛋白表达量显着上调2.32倍。在胆脂瘤上皮的基底层和基底上层中观察到的阳性HB-EGF免疫染色明显强于正常耳后皮肤。同时发现HB-EGF蛋白表达与骨吸收程度呈明显正相关。
Conclusions: The heparin-binding epidermal growth factor-like growth factor (HB-EGF) plays an essential role in the development and invasiveness of cholesteatoma. This study may help to realize the molecular mechanisms underlying the pathogenesis of cholesteatoma and make HB-EGF a promising target for drug intervention of cholesteatoma.Objective: To detect HB-EGF expression in human surgical specimens of acquired middle ear cholesteatoma and analyze its functional role as a regulator of epithelial keratinocytes hyperproliferation.Methods: A total of 34 patients who underwent surgical treatment for middle ear cholesteatoma were recruited in the study. The mRNA and protein expression of HB-EGF in middle ear cholesteatoma tissues and normal postauricular skin tissues was investigated by real-time quantitative reverse-transcription-polymerase chain reaction (RT-qPCR), immunohistochemical staining, and western blot. The correlation between bone resorption degree and HB-EGF expression was also analyzed.Results: On average, compared with normal postauricular skin, expression of HB-EGF mRNA in the cholesteatoma epithelium was significantly elevated 2.41-fold by RT-qPCR, and HB-EGF protein significantly upregulated 2.32-fold by western blot. Positive HB-EGF immunostaining observed in the basal and suprabasal layers of cholesteatoma epithelium was significantly stronger than in normal postauricular skin. Meanwhile, an obviously positive correlation between HB-EGF protein expression and bone resorption degree was discovered.