Is red blood cell rheology preserved during routine blood bank storage?
Is red blood cell rheology preserved during routine blood bank storage?
复制标题
DOI：
10.1111/j.1537-2995.2009.02521.x
复制
发表时间：
2010-04-01
期刊：
影响因子：
2.9
通讯作者：
van Oeveren, Willem
中科院分区：
文献类型：
--
作者：
Henkelman, Sandra;Dijkstra-Tiekstra, Margriet J.;van Oeveren, Willem
背景：在4 ℃下储存超过2周的红细胞（RBC）单位目前被认为质量受损。这种观点主要是基于改变的RBC流变特性（即，增强的聚集性、降低的变形性和升高的内皮细胞相互作用）。在这项研究中，leukoreduced红细胞单位的流变学特性和细胞变量，在常规血库储存在盐水腺嘌呤葡萄糖甘露醇，studiedesignandMETHODS：10 leukoreduced红细胞单位储存在血库7周，在4摄氏度。红细胞的聚集性，变形性，和其他相关variables.RESULTS：红细胞聚集性显着降低后，第一周的存储，但在随后的几周内恢复。7周后，聚集性轻微但显著降低（46.9 +/- 2.4-44.3 +/- 2.2聚集指数）。在储存期间，渗透脆性没有显著增强（0.47 +/- 0.01%磷酸盐缓冲盐水），并且在3.9 Pa剪切应力下的变形性没有显著降低（0.36 +/- 0.01伸长指数[EI]）。50 Pa时的变形能力降低（0.58 +/- 0.01-0.54 +/- 0.01 EI），但仍保持在参考值（0.53 +/- 0.04）范围内。在5个星期的存储，三磷酸腺苷减少了54%，而平均细胞体积，pH值，平均细胞血红蛋白浓度最低限度地affected.CONCLUSIONS：红细胞的生化和物理变化在存储过程中最低限度地影响红细胞的聚集和变形的能力，即使经过长时间的存储。在常规血库储存7周期间，去白细胞红细胞单位的流变学特性保持良好。
BACKGROUND:Red blood cell (RBC) units stored for more than 2 weeks at 4 degrees C are currently considered of impaired quality. This opinion has primarily been based on altered RBC rheologic properties (i.e., enhanced aggregability, reduced deformability, and elevated endothelial cell interaction), during prolonged storage of nonleukoreduced RBC units. In this study, the rheologic properties and cell variables of leukoreduced RBC units, during routine blood bank storage in saline-adenine-glucose-mannitol, were investigated.STUDY DESIGN AND METHODS:Ten leukoreduced RBC units were stored at the blood bank for 7 weeks at 4 degrees C. RBCs were tested weekly for aggregability, deformability, and other relevant variables.RESULTS:RBC aggregability was significantly reduced after the first week of storage but recovered during the following weeks. After 7 weeks aggregability was slightly, but significantly, reduced (46.9 +/- 2.4-44.3 +/- 2.2 aggregation index). During storage the osmotic fragility was not significantly enhanced (0.47 +/- 0.01% phosphate-buffered saline) and the deformability at shear stress of 3.9 Pa was not significantly reduced (0.36 +/- 0.01 elongation index [EI]). The deformability at 50 Pa was reduced (0.58 +/- 0.01-0.54 +/- 0.01 EI) but remained within reference values (0.53 +/- 0.04). During 5 weeks of storage, adenosine triphosphate was reduced by 54% whereas mean cell volume, pH, and mean cell hemoglobin concentration were minimally affected.CONCLUSIONS:RBC biochemical and physical alterations during storage minimally affected the RBC ability to aggregate and deform, even after prolonged storage. The rheologic properties of leukoreduced RBC units were well preserved during 7 weeks of routine blood bank storage.