A multiplexed protein microarray for the simultaneous serodiagnosis of human immunodeficiency virus/hepatitis C virus infection and typing of whole blood
A multiplexed protein microarray for the simultaneous serodiagnosis of human immunodeficiency virus/hepatitis C virus infection and typing of whole blood
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DOI：
10.1016/j.ab.2008.07.017
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发表时间：
2008-11-01
影响因子：
2.9
通讯作者：
Campbell, Colin J.
中科院分区：
文献类型：
--
作者：
Burgess, Stewart T. G.;Kenyon, Fiona;Campbell, Colin J.
所有献血者的血液样本必须在输血前进行检测，以确定献血者的血型。标准检测方案要求对重要的血源性病原体进行检测，如丙型肝炎、梅毒、B型肝炎和人类免疫缺陷病毒。我们已经证明了蛋白质微阵列可以从同一供体血液样本中同时检测全血和重要病原体抗体的概念。收集的数据表明，该阵列能够准确地对血液样本进行分型，同时还能检测出抗人类免疫缺陷病毒和丙型肝炎病毒抗体的存在。总之，我们已经成功开发了一个能够对人类全血样本进行分型的平台，同时检测人类免疫缺陷病毒/丙型肝炎病毒特异性抗体的存在。该系统的主要优点是其可扩展性与额外的测定，例如，恒河猴分型和梅毒和/或乙型肝炎B病毒检测，以及该测定的适应性，以更高的通量分析，目前16个单独的样品每个载玻片，但容易扩展到96孔格式。(C)2008年爱思唯尔公司All rights reserved.
All donor blood samples must be tested pretransfusion to determine the donor blood type. Standard testing protocols require that assays be performed for important bloodborne pathogens such as hepatitis C, syphilis, hepatitis B, and human immunodeficiency virus. We have demonstrated proof of the concept that a protein microarray can type whole blood and detect antibody to significant pathogens simultaneously from the same donor blood sample. The data collected demonstrate the ability of the array to accurately type blood samples while also detecting the presence of antibodies against both human immunodeficiency virus and hepatitis C virus. In conclusion, we have successfully developed a platform capable of typing human whole blood samples, while at the same time testing for the presence of antibodies specific for human immunodeficiency virus/hepatitis C virus. The major benefits of this system are its amenability to expansion with additional assays, for example, rhesus typing and syphilis and/or hepatitis B virus detection, and also the adaptability of the assay to higher-throughput analysis, currently 16 individual samples per slide, but readily expandable to a 96-well format. (C) 2008 Elsevier Inc. All rights reserved.