Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs.
Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs.
复制标题
与使用自我收集的阴道拭子相比，实时PCR可改善阴道感染的检测。
DOI：
10.1080/10647440500068248
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发表时间：
2005-09
影响因子：
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通讯作者：
Wingood, G M
中科院分区：
文献类型：
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作者：
Caliendo, A M;Jordan, J A;Green, A M;Ingersoll, J;Diclemente, R J;Wingood, G M
目的：比较实时聚合酶链反应（PCR）检测与肉汤培养法检测阴道毛滴虫的效果。方法：从参加HIV-1预防项目的青少年和年轻成年非洲裔美国妇女中获得自我收集的阴道拭子。T.使用InPouch TV系统进行迷走神经培养。使用BDProbeTec ET Culturette Direct Dry Swab系统采集用于实时PCR测定的样品，并在实验室开发的测定中进行检测，该测定针对基因组的重复序列。在实时PCR检测中培养阴性和阳性的不一致样品在靶向T.迷走神经基因组、18 S核糖体DNA基因。研究结果：在通过培养和实时PCR检测的524份标本中，36份为培养阳性，54份在实时PCR检测中为阳性; 18份不一致标本中的16份在确证性PCR检测中也为阳性。使用改良的金标准培养阳性或两种PCR检测均阳性，实时PCR检测的灵敏度为100%，特异性为99.6%，而培养的灵敏度为69.2%，特异性为100%。结论：建立的实时荧光定量PCR方法灵敏度高，特异性强。阴道拭子样本的阴道拭子DNA。使用BDProbeTec干拭子系统进行实时PCR检测的能力允许检测沙眼衣原体、淋病奈瑟菌和T。一个样本的迷走神经
OBJECTIVE: To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection of Trichomonas vaginalis using self-collected vaginal swabs. METHODS: Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs. T. vaginalis culture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real-time PCR assay were tested in a confirmatory PCR which targeted a different region of the T. vaginalis genome, the18S ribosomal DNA gene. RESULTS: Of the 524 specimens tested by both culture and real-time PCR, 36 were culture positive and 54 were positive in the real-time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a modified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real-time PCR assay was 100% and the specificity was 99.6%, whereas culture had a sensitivity of 69.2% and a specificity of 100%. CONCLUSIONS: The real-time PCR assay was sensitive and specific for the detection of T. vaginalis DNA from self-collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real-time PCR testing allowed for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and T. vaginalis from a single specimen.