BIOLOGICAL AND CHEMICAL STUDIES ON MYCOBACTERIA - RELATIONSHIP OF COLONY MORPHOLOGY TO MYCOSIDE CONTENT FOR MYCOBACTERIUM KANSASII AND MYCOBACTERIUM FORTUITUM
BIOLOGICAL AND CHEMICAL STUDIES ON MYCOBACTERIA - RELATIONSHIP OF COLONY MORPHOLOGY TO MYCOSIDE CONTENT FOR MYCOBACTERIUM KANSASII AND MYCOBACTERIUM FORTUITUM
复制标题
DOI：
10.1128/jb.82.4.517-527.1961
复制
发表时间：
1961-01-01
影响因子：
3.2
通讯作者：
SMITH, DW
中科院分区：
文献类型：
--
作者：
FREGNAN, GB;RANDALL, HM;SMITH, DW
使用合适的技术和适当的培养基，可以显示堪萨斯分枝杆菌（光致发色团）和偶然分枝杆菌菌株的独特和统一的菌落特征，这些菌株新鲜分离自患者或土壤，或在我们的储备培养物收集中保存数年。已经提出了新的符号来表示这些菌落类型。已证明菌落形态与给定菌株中存在的特定真菌苷密切相关; kansasii菌株在每种情况下都显示出菌落类型K和真菌苷A，以及M。偶然分离的菌株表现为F型菌落和真菌苷F。需要注意所使用的技术和媒介的重要性。在含有5 - 10%CO2的空气中培养，菌落形态没有变化，尽管这改善了生长。在甘油、人类分枝杆菌菌株的脂质或碳酸氢钠的存在下，分枝杆菌的生长更好，但菌落形式的特异性丧失。
Using a suitable technique and an adequate medium it was possible to show a unique and uniform type of colony characteristic for Mycobacterium kansasii (photochromogen) and for Mycobacterium fortuitum strains freshly isolated either from patients, or from the soil, or kept in our stock culture collection for several years. New symbols have been proposed to represent these colony types. It was demonstrated that colony morphology is closely related to the specific mycoside present in a given strain; for example, M. kansasii strains showed in each instance colony type K and mycoside A, and M. fortuitum strains showed colony type F and mycoside F. Attention is called to the importance of the technique and the medium used. No change in colony morphology resulted from incubation in the presence of air containing 5 to 10% CO2, although this improved growth. Better growth of myobacteria occurred in the presence of glycerol, or lipids of a human strain of mycobacteria, or sodium bicarbonate, but the specificity of colony form was lost.