Label-free biosensor: a novel phage-modified Light Addressable Potentiometric Sensor system for cancer cell monitoring.
Label-free biosensor: a novel phage-modified Light Addressable Potentiometric Sensor system for cancer cell monitoring.
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DOI：
10.1016/j.bios.2007.01.018
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发表时间：
2007-06
影响因子：
12.6
通讯作者：
Yunfang Jia;Ming Qin;Hongkai Zhang;Wencheng Niu;Xiao Li;Likai Wang;Xin Li;Yunpeng Bai;Youjia Cao;Xizeng Feng
中科院分区：
文献类型：
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作者：
Yunfang Jia;Ming Qin;Hongkai Zhang;Wencheng Niu;Xiao Li;Likai Wang;Xin Li;Yunpeng Bai;Youjia Cao;Xizeng Feng
在临床医学中，早期诊断已成为影响肿瘤治疗效果的最重要因素。在这里，我们提出了一种基于噬菌体修饰的光寻址电位传感器(phage-LAPS)检测癌症标志物和癌细胞的新方法。噬菌体共价固定在Si3N4芯片表面。该噬菌体LAPS在0.04~400 nm浓度范围内检测人再生性肝细胞3磷酸酶(hPRL-3)，在0~105ml浓度范围内检测乳腺癌细胞(MDAMB231)。最大响应信号分别约为10和60μV。结果表明，该系统比肿瘤生物标记物更适用于癌细胞的检测。这项工作可能在肿瘤的临床检测和噬菌体-细胞相互作用的研究中具有潜在的应用价值。
Early diagnosis has become the most important factor influencing the cancer's curing efficiency in clinical medicine. Here we present a new way for detection of cancer markers and cancer cells based on phage-modified Light Addressable Potentiometric Sensor (phage-LAPS). Phages were immobilized on the Si3N4chip surfaces covalently. Using the back-illuminating system, this phage-LAPS is tested to detect human phosphatase of regenerating liver-3 (hPRL-3) in the concentrations of 0.04–400nM, and the mammary adenocarcinoma cell (MDAMB231) in the concentrations of 0–105mL. The maximum responding signal is about 10 and 60μV, respectively. The results demonstrated that the system was more applicable to detection of cancer cell than that of cancer biomarkers. This work might show potential application in clinic assays of cancer and the study of phage–cell interaction.