Inhibition of SV40-induced cellular DNA synthesis by microinjection of monoclonal antibodies.
Inhibition of SV40-induced cellular DNA synthesis by microinjection of monoclonal antibodies.
复制标题
通过显微注射单克隆抗体抑制 SV40 诱导的细胞 DNA 合成。
DOI：
10.1016/0042-6822(83)90379-3
复制
发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Baserga,R
中科院分区：
文献类型：
--
作者：
Mercer,WE;Nelson,D;Hyland,JK;Croce,CM;Baserga,R
已使用杂合腺病毒-SV 40病毒和克隆缺失突变体的手动显微注射确定了由一组单克隆抗体识别的SV 40大T抗原分子的区域。此外，还研究了显微注射入细胞核的单克隆抗体如何影响T抗原编码基因刺激细胞DNA合成的能力。将识别大T的-000H末端一半的单克隆抗体Pab 14与质粒pCl-1一起共显微注射到静止细胞中。该质粒仅包含T抗原编码基因的从核苷酸残基120逆时针延伸到核苷酸残基4002的部分，并产生分子量为33，000的截短的T抗原，并且在-COON末端侧缺失最后435个氨基酸。单克隆抗体Pab 14不抑制pCl-1显微注射引起的细胞DNA合成的刺激，尽管它确实抑制pSV 2G显微注射诱导的细胞DNA合成，pSV 2G是一种含有SV 40的整个T抗原编码基因的重组质粒。
The region of the SV40 large T-antigen molecule recognized by a panel of monoclonal antibodies has been determined using hybrid Adeno-SV40 viruses, and manual microinjection of cloned deletion mutants. In addition, an investigation was made of how monoclonal antibodies microinjected into the nucleus can affect the ability of the T-antigen coding gene to stimulate cell DNA synthesis. The monoclonal antibody Pab 14, that recognized the -000H terminal half of large T, was comicroinjected into quiescent cells together with plasmid pCl-1. This plasmid contains only that part of the T-antigen coding gene that extends from nucleotide residue 120, counterclockwise to nucleotide residue 4002, and makes a truncated T antigen 33,000 in molecular weight and missing the last 435 amino acids on the -COON terminal side. Monoclonal antibody Pab 14 did not inhibit the stimulation of cellular DNA synthesis caused by microinjection of pCl-1, although it did inhibit cell DNA synthesis induced by microinjection of pSV2G, a recombinant plasmid that contains the entire T-antigen coding gene of SV40.