Label-Free DNA Biosensors Based on Functionalized Carbon Nanotube Field Effect Transistors
Label-Free DNA Biosensors Based on Functionalized Carbon Nanotube Field Effect Transistors
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DOI：
10.1021/nl8025604
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发表时间：
2009-02-01
期刊：
影响因子：
10.8
通讯作者：
Bokor, Jeffrey
中科院分区：
文献类型：
--
作者：
Martinez, Maria Teresa;Tseng, Yu-Chih;Bokor, Jeffrey
利用碳纳米管晶体管阵列检测DNA杂交。一种新的方法，以确保特异性吸附的DNA纳米管的开发。合成了聚合物聚（甲基丙烯酸甲酯（0.6）-co-聚（乙二醇）甲基丙烯酸酯（0.15）-co-N-琥珀酰亚胺基甲基丙烯酸酯（0.25）），并将其非共价键合到纳米管上。然后将胺化的单链DNA共价连接到聚合物上。杂交后，观察到关键晶体管参数的统计学显著变化。杂交DNA捕获电子和空穴，可能是由碱基对的电荷捕获性质引起的。
A carbon nanotube transistor array was used to detect DNA hybridization. A new approach to ensure specific adsorption of DNA to the nanotubes was developed. The polymer poly (methylmethacrylate(0.6)-co-poly(ethyleneglycol)methacrylate(0.15)-co-N-succinimidyl methacrylate(0.25)) was synthesized and bonded noncovalently to the nanotube. Aminated single-strand DNA was then attached covalently to the polymer. After hybridization, statistically significant changes were observed in key transistor parameters. Hybridized DNA traps both electrons and holes, possibly caused by the charge-trapping nature of the base pairs.