Patterns of mRNA expression for matrix molecules and growth factors in flexor tendon injury: Differences in the regulation between tendon and tendon sheath
Patterns of mRNA expression for matrix molecules and growth factors in flexor tendon injury: Differences in the regulation between tendon and tendon sheath
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DOI：
10.1016/j.jhsa.2006.06.011
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发表时间：
2006-10-01
影响因子：
1.9
通讯作者：
Wiig, Monica
中科院分区：
文献类型：
--
作者：
Berglund, Maria;Reno, Carol;Wiig, Monica
目的：肌腱损伤，尤其是屈肌腱损伤，在愈合后可能因粘连形成和其他并发症而导致功能丧失。本研究的目的是增进我们对肌腱和腱鞘愈合过程的理解，以便开发影响愈合过程的方法，从而在未来改善肌腱修复的效果。
方法：在屈肌腱损伤的兔模型中，于术后3、6、12和24天采集组织（每组n = 6）。提取RNA后，使用逆转录聚合酶链反应测量肌腱和腱鞘中相关基因的信使RNA（mRNA）水平。将损伤后不同时间点相关分子的mRNA水平与肌腱和腱鞘的未损伤对照组进行比较。
结果：损伤初期，胶原蛋白表达发生变化，肌腱和腱鞘中Ⅲ型mRNA水平显著升高，而Ⅰ型胶原蛋白的mRNA水平仅在后期在腱鞘中升高。聚集蛋白聚糖和多功能蛋白聚糖的mRNA水平在两种组织中均升高，但变化的时间方面不同。双糖链蛋白聚糖和基膜聚糖的mRNA水平在整个所检测的愈合期间均上调，而核心蛋白聚糖的mRNA水平在肌腱中在整个期间显著降低，在腱鞘中降低程度较轻。碱性成纤维细胞生长因子和转化生长因子β的mRNA水平在肌腱损伤后升高，但在腱鞘中未升高。相反，结缔组织生长因子的mRNA水平在整个评估期间在两种组织中未改变或降低。
结论：兔屈肌腱和腱鞘损伤后的愈合遵循一种可重复的基因表达模式；然而，肌腱中的模式与腱鞘中的模式非常不同。这些发现表明，为改善这些组织的愈合而开发的干预措施必须解决这些差异，因为它们可能会影响结果。
Purpose: Injuries to tendons, particularly flexor tendons, can lead to loss of function after healing due to adhesion formation and other complications. The aim of this study was to increase our understanding of the healing process in tendons and tendon sheaths to develop methods to affect the healing process and improve the outcome of tendon repair in the future.Methods: In a rabbit model of flexor tendon injury, tissues were harvested 3, 6, 12, and 24 days after surgery (n = 6 for each group). After RNA extraction, messenger RNA (mRNA) levels for relevant genes in tendon and tendon sheaths were measured using the reverse transcription polymerase chain reaction. Messenger RNA levels for a subset of relevant molecules at different time points after injury were compared with those of uninjured controls for tendons and tendon sheaths.Results: Initially after injury, there was a shift in collagen expression with a marked increase in type III mRNA levels in both the tendon and tendon sheath, whereas those for collagen I increased only in the sheath at later time points. Aggrecan and versican mRNA levels were increased in both tissues, but temporal aspects of the changes were-different. The mRNA levels for biglycan and lumican were all upregulated throughout the healing interval examined, whereas those for decorin were significantly decreased throughout in the tendon more so than the sheath. The mRNA levels for basic fibroblastic growth factor and transforming growth factor beta were elevated after injury in the tendon but not in the sheath. In contrast, mRNA levels for connective tissue growth factor were unaltered or decreased in both tissues throughout the interval assessed.Conclusions: Healing after injury to the rabbit flexor tendon and tendon sheath follow a reproducible pattern of gene expression; however, the pattern in the tendon is very different from that in the sheath. These findings indicate that interventions developed to improve healing of these tissues will have to address these differences, because they will likely affect the outcomes.