NERVE GROWTH FACTOR FROM MOUSE SUBMAXILLARY GLAND - AMINO ACID SEQUENCE
NERVE GROWTH FACTOR FROM MOUSE SUBMAXILLARY GLAND - AMINO ACID SEQUENCE
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DOI：
10.1073/pnas.68.10.2417
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
BRADSHAW, RA
中科院分区：
文献类型：
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作者：
ANGELETT.RH;BRADSHAW, RA
测定了雄性小鼠颌下腺2.5S神经生长因子的全氨基酸序列。来自胰蛋白酶，胰凝乳蛋白酶，therapeutic和消化酶消化的S-羧甲基，S-氨乙基，和天然生长因子的肽的明确对齐表明，主要亚基由118个氨基酸组成，氨基末端丝氨酸和羧基末端精氨酸。根据一级序列计算，该亚基的分子量为13，259。因此，由两个亚基组成的天然蛋白质的分子量为26，518。这些值，以及最终的氨基酸组成，是在良好的协议，通过直接测量与未消化的生长因子。三个二硫键的排列，由消化性和治疗性二硫键的组合确定，是I-IV，II-V和III-VI。后两对位于14个氨基酸的闭环中，这是由于半胱氨酸残基V和VI在线性序列中仅被单个残基分开的事实。侧链酰胺的分配表明，11个天冬氨酸残基中的7个和8个谷氨酸残基中的2个作为酰胺存在。带电残基的这种分布与观察到的等电点9.3完全一致。
The complete amino-acid sequence of the 2.5S nerve growth factor from male-mouse submaxillary glands has been determined. The unambiguous alignment of peptides derived from tryptic, chymotryptic, thermolytic, and peptic digestion ofS-carboxymethyl-,S-aminoethyl-, and native growth factor indicates that the primary subunit is composed of 118 amino acids, with amino-terminal serine and carboxyl-terminal arginine. The molecular weight of this subunit, calculated from the primary sequence, is 13,259. Thus, the native protein, which is composed of two of the subunits, has a molecular weight of 26,518. These values, as well as the final amino-acid composition, are in excellent agreement with those determined by direct measurement with undigested growth factor. The alignment of the three disulfide bonds, determined from a combination of peptic and thermolytic digestions, is I-IV, II-V, and III-VI. The latter two pairs are located in a closed loop of 14 amino acids, by virtue of the fact that half-cystinyl residues V and VI are separated by only a single residue in the linear sequence. Assignment of the side-chain amides showed that 7 of 11 aspartic acid residues and 2 of 8 glutamic acid residues are present as amides. This distribution of charged residues is entirely consistent with the observed isoelectric point of 9.3.