The influence of simulated body fluid composition on carbonated hydroxyapatite formation
The influence of simulated body fluid composition on carbonated hydroxyapatite formation
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模拟体液成分对碳酸羟基磷灰石形成的影响
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
L. Sanda
中科院分区：
文献类型：
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作者：
A. Helebrant;L. Jonášová;L. Sanda
采用体外模拟体液（SBF）对骨和牙种植体材料进行了测试。大多数使用的SBF的组成与血浆的不同之处在于Cl -离子含量高，hco3 -离子含量低。考虑到骨状磷灰石的组成，其中含有碳酸盐离子，测试结果可能会受到这种差异的影响。采用142Na +、5K +、2.5Ca 2+、1Mg 2+、1so4.2 -、1HPO 4.2 -、136（Cl - +HCO 3 -）的浸出液对na2o - cao - sio2 - p 2o - 5体系玻璃进行浸出。hco3 -的含量在5 ~ 27 mmol/l之间。2天后，所有溶液中的磷浓度都下降，表明在玻璃表面形成了磷酸盐层。x射线衍射（XRD）证实了不同溶液中磷灰石层形成程度的不同。XRD峰的强度似乎更受初始溶液过饱和的影响，而不是相互作用过程中过饱和值的影响。这表明成核可能是磷灰石析出的控制机制。实验和过饱和实验结果均表明，增加hco3 -离子量的SBF更适合于体外生物活性物质的检测。
The materials for bone and dental implants are tested in vitro using simulated body fluid (SBF). The composition of most used SBF differs from that of blood plasma by high content of Cl - ions and lower content of HCO 3 - ions. Considering the composition of bone-like apatite, which contains carbonate ions, the test results could be influenced by this difference. The glass of system Na 2 O-CaO-SiO 2 -P 2 O 5 was leached in fluids of composition (in mmol/l): 142Na + , 5K + , 2.5Ca 2+ , 1Mg 2+ , 1SO 4 2- , 1HPO 4 2- , 136(Cl - +HCO 3 - ). The content of HCO 3 - ranged between 5 and 27 mmol/l. The decrease of phosphorus concentration was observed after 2 days in all solutions indicating the creation of phosphate layer on the glass surface. X-ray diffraction (XRD) confirmed the various extent of apatite layer creation in different solutions. The intensity of XRD peaks seems to be more influenced by the initial solution supersaturation than by the supersaturation values during the interaction. This fact indicates that the nucleation could be the control mechanism of apatite precipitation. Both experimental and supersaturation results indicate that the SBF with increased amount of HCO 3 - ions is more appropriate and sensible to in vitro testing of bioactive materials.