Experimental oesophagitis in the rat is associated with decreased voluntary movement
Experimental oesophagitis in the rat is associated with decreased voluntary movement
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DOI：
10.1111/j.1365-2982.2008.01221.x
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发表时间：
2009-03-01
影响因子：
3.5
通讯作者：
Koseki, J.
中科院分区：
文献类型：
--
作者：
Miwa, H.;Oshima, T.;Koseki, J.
关于消化不良症状产生的机制已经引起了越来越多的兴趣。然而，还没有在动物中开发这些症状的评估系统。在这项研究中，我们研究了大鼠的自主运动是否可以作为评估反流性食管炎内脏症状的一种措施。采用大鼠慢性酸反流性食管炎模型，测量糜烂面积。奥美拉唑给予食管炎大鼠10天。通过红外传感器测量自主运动的量。还使用发射电子显微镜测量食管上皮细胞内间隙。通过蛋白质印迹法检查NP-40可溶性和不溶性的密封蛋白级分。食管炎模型组大鼠自主运动能力明显低于假手术组（P < 0.01）。虽然奥美拉唑减少了糜烂的大小，但对自主运动总量无显著影响（r =-0.033，P = 0.916）。在食管炎模型大鼠中细胞内空间显著扩张，并且在NP-40不溶性级分中密蛋白-3显示出显著较低的相对量。奥美拉唑能显著增加食管炎模型大鼠的自主运动量和不溶性部分中claudin-3的相对含量（P < 0.05）。细胞间隙扩张和claudin-3水平降低可能与食管炎模型大鼠的自主运动有关。食管炎模型大鼠自主运动减少可反映内脏症状，可作为慢性腹部症状的指标。
Growing interest has arisen regarding the mechanism of dyspeptic symptom generation. However, no evaluation system of these symptoms in animals has been developed. In this study, we examined whether voluntary movement of rats could be a measure to assess visceral symptoms of reflux oesophagitis. A chronic acid reflux oesophagitis model was made using rats, and the size of erosions was measured. Omeprazole was administered to the oesophagitis rats for 10 days. The amount of voluntary movement was measured by an infrared sensor. Intracellular spaces in oesophageal epithelium were also measured using a emission electron microscope. NP-40 soluble and insoluble fractions of claudins were examined by Western blot. Voluntary movement was significantly lower in the oesophagitis model rats than in the sham-operated rats (P < 0.01). Although omeprazole reduced the size of erosions, it did not significantly affect the total amount of voluntary movement (r = -0.033, P = 0.916). Intracellular spaces were significantly dilated in the oesophagitis model rats and claudin-3 showed a significantly lower relative quantity in the NP-40 insoluble fraction. Omeprazole significantly increased voluntary movement of oesophagitis model rats and the relative quantity of claudin-3 in the insoluble fraction (P < 0.05). Dilated intercellular spaces and the lower level of claudin-3 may relate to the voluntary movement of oesophagitis model rats. Decreases in voluntary movement of oesophagitis model rats may reflect visceral symptoms and be able to serve as an index of chronic abdominal symptoms.