Detection of Inflammation in Vivo by Surface-Enhanced Raman Scattering Provides Higher Sensitivity Than Conventional Fluorescence Imaging
Detection of Inflammation in Vivo by Surface-Enhanced Raman Scattering Provides Higher Sensitivity Than Conventional Fluorescence Imaging
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DOI：
10.1021/ac3006445
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发表时间：
2012-07-17
影响因子：
7.4
通讯作者：
Garside, Paul
中科院分区：
文献类型：
--
作者：
McQueenie, Ross;Stevenson, Ross;Garside, Paul
检测炎症变化是早期诊断和治疗一些自身免疫性、感染性和转移性疾病的关键目标。虽然表面增强拉曼散射（SERS）能够提供无创的、足够深度的体内成像来实现这一目标，但这种方法尚未被用于炎症研究。具有sers活性的纳米颗粒被编码为独特的拉曼信号，在广泛的条件和刺激下受到保护。为了检测早期炎症，将含有拉曼活性分子的金纳米颗粒簇与细胞间粘附分子1- (ICAM-1-)特异性单克隆抗体偶联。SERS可以无创测量体内ICAM-1的表达，灵敏度是双光子荧光的两倍。这是第一次将SERS用于体内炎症检测，并且是用于无创、下一代体内成像的不断增长的方法工具包的重大进步。
The detection of inflammatory changes is a key aim for the early diagnosis and treatment of several autoimmune, infectious, and metastatic diseases. While surface-enhanced Raman scattering (SERS) has the capability to provide noninvasive, in vivo imaging at sufficient depth to achieve this goal, this approach has not been exploited in the study of inflammation. SERS-active nanoparticles were coded with a unique Raman signal that was protected under a wide range of conditions and stimuli. To detect early-stage inflammation, gold nanoparticle clusters containing Raman-active molecules were conjugated to intercellular adhesion molecule 1- (ICAM-1-) specific monoclonal antibodies. SERS allowed noninvasive measurement of ICAM-1 expression in vivo with twice the sensitivity of two photon fluorescence. This is the first time SERS has been used for in vivo detection of inflammation and is a major advance in the ever-growing toolkit of approaches for use in noninvasive, next generation in vivo imaging.