Dynamic Expression of Sox2, Gata3, and Prox1 during Primary Auditory Neuron Development in the Mammalian Cochlea.
Dynamic Expression of Sox2, Gata3, and Prox1 during Primary Auditory Neuron Development in the Mammalian Cochlea.
复制标题
哺乳动物耳蜗中原发性听觉神经元发育过程中SOX2，GATA3和Prox1的动态表达。
DOI：
10.1371/journal.pone.0170568
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Dabdoub A
中科院分区：
文献类型：
--
作者：
Nishimura K;Noda T;Dabdoub A
初级听觉神经元（PANS）将哺乳动物内耳的耳蜗感觉毛细胞与脑干的耳蜗核神经元连接起来。PAN从耳囊的前神经感觉域分层的成神经细胞发育，并保持成熟，直到出生后听力开始。有两种类型的PAN：I型，其神经支配内毛细胞（IHC），和II型，其神经支配外毛细胞（OHC）。这些神经元周围的神经胶质细胞起源于神经嵴细胞，并迁移到螺旋神经节。已知几种转录因子调节PAN的发育和分化。在这里，我们系统地研究了5个转录因子的时空表达：Sox 2，Sox 10，Gata 3，Mafb和Prox 1从早期分层在胚胎天（E）10.5成人。我们发现Sox 2和Sox 10最初在耳囊（E10.5）的前神经感觉细胞中表达。到E12.75，Sox 2和Sox 10在发育中的PAN中均下调;然而，Sox 2表达在出生前后的神经元中短暂增加。此外，Sox 2和Sox 10继续在螺旋神经节胶质细胞中表达。我们还表明，Gata 3和Prox 1首先在所有发育中的神经元中表达，然后随着它们的成熟，Gata 3和Mafb在I型PAN中的表达减少，Prox 1在II型PAN中的表达减少。此外，我们描述了两个亚型的II型神经元的基础上的外周蛋白表达。这些结果表明，Sox 2，Gata 3和Prox 1在神经发生以及PAN的成熟过程中发挥作用。
Primary auditory neurons (PANs) connect cochlear sensory hair cells in the mammalian inner ear to cochlear nucleus neurons in the brainstem. PANs develop from neuroblasts delaminated from the proneurosensory domain of the otocyst and keep maturing until the onset of hearing after birth. There are two types of PANs: type I, which innervate the inner hair cells (IHCs), and type II, which innervate the outer hair cells (OHCs). Glial cells surrounding these neurons originate from neural crest cells and migrate to the spiral ganglion. Several transcription factors are known to regulate the development and differentiation of PANs. Here we systematically examined the spatiotemporal expression of five transcription factors: Sox2, Sox10, Gata3, Mafb, and Prox1 from early delamination at embryonic day (E) 10.5 to adult. We found that Sox2 and Sox10 were initially expressed in the proneurosensory cells in the otocyst (E10.5). By E12.75 both Sox2 and Sox10 were downregulated in the developing PANs; however, Sox2 expression transiently increased in the neurons around birth. Furthermore, both Sox2 and Sox10 continued to be expressed in spiral ganglion glial cells. We also show that Gata3 and Prox1 were first expressed in all developing neurons, followed by a decrease in expression of Gata3 and Mafb in type I PANs and Prox1 in type II PANs as they matured. Moreover, we describe two subtypes of type II neurons based on Peripherin expression. These results suggest that Sox2, Gata3 and Prox1 play a role during neurogenesis as well as maturation of the PANs.