Intracytoplasmic localization of cathepsin D reflects the invasive potential of gastric carcinoma
Intracytoplasmic localization of cathepsin D reflects the invasive potential of gastric carcinoma
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组织蛋白酶 D 的胞质内定位反映了胃癌的侵袭潜力
DOI：
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发表时间：
2001
影响因子：
6.3
通讯作者：
T. Fujimori
中科院分区：
文献类型：
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作者：
H. Kashida;H. Kawamata;K. Ichikawa;K. Morita;J. Imura;T. Fujimori
背景本研究旨在探讨组织蛋白酶D在胃癌侵袭中的作用。方法.组织蛋白酶D免疫组化染色进行了29个早期胃腺癌（乳头状或管状腺癌）和15个胃腺瘤，其相邻的非肿瘤性胃粘膜的样本。我们将组织蛋白酶D免疫染色的模式分为四种类型：A型，顶端部分的细颗粒染色; B型，顶端部分的强烈粗颗粒染色; C型，基底部分的细颗粒染色;和D型，整个细胞质的弥漫性颗粒染色。结果所有非肿瘤性粘膜均显示顶端胞质分布模式（A型或B型）。然而，20%（2/10）的低级别胃腺瘤和60%（3/5）的高级别胃腺瘤显示异常染色模式，即，C型和D型。此外，82%（9/11）的明确粘膜内胃腺癌和大多数具有粘膜下浸润的胃腺癌[83%（15/18）的粘膜部分和100%（14/14）的粘膜下浸润部分]显示异常染色模式（C型和D型）。有趣的是，大多数侵入间质和淋巴管的癌细胞均呈D型染色。结论.这些结果表明，组织蛋白酶D的异常胞浆染色模式可能反映了胃癌细胞的侵袭能力。
Background. The present study was undertaken to investigate the role of cathepsin D in the invasiveness of human gastric cancer. Methods. Immunohistochemical cathepsin D staining was performed in samples from 29 early gastric adenocarcinomas (papillary or tubular adenocarcinoma) and 15 gastric adenomas, and their adjacent nonneoplastic gastric mucosa. We classified the patterns of cathepsin D immunostaining into four types; type A, fine granular staining in the apical portion; type B, intense coarse granular staining in the apical portion; type C, fine granular staining in the basal portion; and type D, diffuse granular staining throughout the cytoplasm. Results. All of the nonneoplastic mucosa showed an apical cytoplasmic distribution pattern (type A or type B). However, 20% (2/10) of low-grade gastric adenomas and 60% (3/5) of high-grade gastric adenomas showed an abnormal staining pattern, i.e., types C and D. Moreover, 82% (9/11) definite intramucosal gastric adenocarcinomas, and the majority of gastric adenocarcinomas with submucosal invasion [83% (15/18) of those in the mucosal part and 100% (14/14) of those in the invasive submucosal part] showed an abnormal staining pattern (types C and D). Interestingly, most of the carcinoma cells invading the stroma and lymphatics showed the type D staining pattern. Conclusions. These results indicate that an abnormal cytoplasmic staining pattern of cathepsin D may reflect the invasive potential of gastric carcinoma cells.
影响因子：
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作者：
CHEN, WT
通讯作者：
CHEN, WT
M(r) 72,000 基质金属蛋白酶在细胞表面侵袭伪足的结合和定位。
影响因子：
11.2
作者：
Monsky,WL;Kelly,T;Lin,CY;Yeh,Y;Stetler-Stevenson,WG;Mueller,SC;Chen,WT
通讯作者：
Chen,WT