Roles of MITF for development of mast cells in mice: effects on both precursors and tissue environments
Roles of MITF for development of mast cells in mice: effects on both precursors and tissue environments
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DOI：
10.1182/blood-2004-01-0247
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发表时间：
2004-09-15
期刊：
影响因子：
20.3
通讯作者：
Kitamura, Y
中科院分区：
文献类型：
--
作者：
Morii, E;Oboki, K;Kitamura, Y
突变型tg/tg小鼠不表达mi转录因子（MITF），在大多数组织中缺乏肥大细胞。由于MITF在肥大细胞和肥大细胞发育的组织中表达，因此tg/tg小鼠可能在肥大细胞前体和组织环境中均显示异常。我们通过骨髓和皮肤移植来检查这种可能性。当将tg/tg小鼠的骨髓细胞移植到具有正常组织环境的W/W-v小鼠时，在所检查的所有组织中均未出现肥大细胞。当移植到tg/tg受体时，W/W-v小鼠皮肤中发育中的肥大细胞的数量比移植到正常（+/+）受体时低得多。这些结果表明tg/tg小鼠肥大细胞前体细胞存在缺陷。当+/+小鼠骨髓细胞为细胞时，tg/tg受体的受检组织中肥大细胞的数量显著低于W/W-v受体，表明tg/tg小鼠肥大细胞发育的组织环境存在缺陷。MITF对肥大细胞前体和组织环境的功能至关重要。2004年，美国血液学会公布了移植后发生肥大（C）的数量。
The mutant tg/tg mice, which do not express mi transcription factor (MITF), lack mast cells in most tissues. Since MITF is expressed in both mast cells and tissues where mast cells develop, there is a possibility that the tg/tg mice may show abnormalities in both mast cell precursors and tissue environments. We examined this possibility by bone marrow and skin transplantation. When bone marrow cells of tg/tg mice were transplanted to W/W-v mice that possess normal tissue environment, mast cells did not develop in all tissues examined. The number of developing mast cells in the skin of W/W-v mice was much lower when grafted to tg/tg recipients than when grafted to normal (+/+) recipients. These results indicated that mast cell precursors of tg/tg mice were defective. When bone marrow cells of +/+ mice were cells was significantly lower in examined tissues of tg/tg recipients than in those of W/W-v recipients, suggesting that the tissue environment for mast cell development was defective in tg/tg mice. MITF appeared essential for the function of both mast cell precursors and tissue environments for their development. transplanted, the number of developing mast (C) 2004 by The American Society of Hematology.