A novel DNA tweezers-based nanobiosensor for multiple detections of circulating exosomal microRNAs in breast cancer.
A novel DNA tweezers-based nanobiosensor for multiple detections of circulating exosomal microRNAs in breast cancer.
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DOI：
10.1016/j.ab.2022.114697
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发表时间：
2022-08-15
影响因子：
2.9
通讯作者：
Rahaie, Mahdi
中科院分区：
文献类型：
--
作者：
Agahi, Melika;Rahaie, Mahdi
乳腺癌是女性的常见病，早期诊断并采取预防措施至关重要。最近，循环中的microRNAs已成为癌症的早期生物标志物。MiR-21和miR-155是乳腺癌中两个重要的生物标志物。在这项研究中，为了在一次测试中同时检测两个microRNA，根据特定的G-四链纳米结构的过氧化特性，开发了一种新型的比色纳米生物传感器。该纳米结构将三个DNA寡核苷酸与靶序列自组装后形成DNA纳米钳，并以TMB(2，2‘-氮基-双(3-乙基苯并噻偶氮-6-磺酸))为报告试剂显色。用不同浓度的靶序列测定了该纳米生物传感器的高灵敏度(在缓冲液和血液中)，线性响应范围为0~10 nm，检出限为0.38 nm(R2=0.98)。该方法从缓冲液和血液介质中的非靶序列中准确地检测到靶序列。这些发现表明，纳米生物传感器由于其同时双重检测、简单、响应时间短和成本低而优于大多数以前发表的工作。更重要的是，分析数据便于准确用于临床早期乳腺癌的检测。
Breast cancer is the prevalent disease in women, and diagnosis of it in early stage and takes preventive measures is very critical. Recently, circulating microRNAs have emerged as promising early biomarkers of cancer. MiR-21 and miR-155 are two significant biomarkers that act as oncomir in breast cancer. In this study, to detect both microRNAs in one test simultaneously, a novel colorimetric nanobiosensor was developed upon the peroxidation property of a specific G-quadruplex nanostructure. The nanostructure forms a DNA Nano-Tweezers after self-assembly of three DNA oligonucleotides with target sequences, and TMB (2, 2'-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid)) is used as a reporter to produce color. The high sensitivity of the nanobiosensor was determined (in buffer and blood) using different concentrations of target sequences with a linear response range from 0 to 10nM, and detection limit of 0.38nM(R2=0.98). The method precisely detected target sequences from non-target sequences in both buffer and blood media. These findings demonstrate, the nanobiosensor is superior to most previous published works due to its simultaneous dual detection, simplicity, low response time, and cost. The analytical data is convenient for accurately use for clinical purposes to detect breast cancer in early stage, more significantly.