Analysis of human brain tissue, brain tumors and tumor cells by infrared spectroscopic mapping
Analysis of human brain tissue, brain tumors and tumor cells by infrared spectroscopic mapping
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DOI：
10.1039/b408934k
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Salzer, R
中科院分区：
文献类型：
--
作者：
Krafft, C;Sobottka, SB;Salzer, R
本研究使用红外光谱、点检测、映射程序来检查来自正常脑标本和来自星形胶质细胞胶质瘤（最常见的人类脑肿瘤）的组织样本。模型系统来源于培养的神经胶质瘤细胞系。正常组织切片的红外光谱通过增加脂质和胆固醇的光谱贡献区分白色物质和灰质。定性地，在低级别和高级别胶质瘤组织切片的IR光谱中发现了相同的差异，这表明随着恶性程度的增加，脑脂质显著减少。而蛋白质和脂质的光谱贡献是相似的胶质瘤细胞和组织的红外光谱，核酸带更强烈的细胞表明更高的增殖活性。对于71个样品的红外光谱图的统计分析，脂质与蛋白质的比例的参数被引入，涉及与脂质作为主要贡献者的CH2对称拉伸带和蛋白质的酰胺I带。由于该参数与从标准组织病理学检查获得的胶质瘤等级相关，因此基于IR光谱映射将其应用于脑组织切片分类。
This study uses infrared (IR) spectroscopic, point detection, mapping procedures to examine tissue samples from normal brain specimens and from astrocytic gliomas, the most frequent human brain tumors. Model systems were derived from cultured glioma cell lines. IR spectra of normal tissue sections distinguished white matter from gray matter by increased spectral contributions from lipids and cholesterol. Qualitatively the same differences were found in IR spectra of low and high grade glioma tissue sections pointing to a significant reduction of brain lipids with increasing malignancy. Whereas spectral contributions of proteins and lipids were similar in IR spectra of glioma cells and tissues, nucleic acid bands were more intense for cells suggesting higher proliferative activities. For statistical analyses of IR spectroscopic maps from 71 samples, a parameter for the lipid to protein ratio was introduced involving the CH2 symmetric stretch band with lipids as main contributors and the amide I band of proteins. As this parameter correlated with the grade of gliomas obtained from standard histopathological examination, it was applied to classify brain tissue sections based on IR spectroscopic mapping.