Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN)

Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN)
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通过幼苗与促进植物生长的根际细菌(假单胞菌菌株 PsJN)的体外共培养增强番茄移植物的黄萎病抗性

DOI:
10.1139/cjm-44-6-528
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发表时间:
1998
影响因子:
2.8
通讯作者:
J. Nowak
J. Nowak
中科院分区:
生物学4区
文献类型:
--
作者:
V. Sharma;J. Nowak

文献摘要

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促进植物生长的根际细菌假单胞菌的潜在利用为提高番茄移栽植株对黄萎病的抗性,对菌株PsJN进行了研究。以感病品种Bonny Best为试材,对黄萎病病菌侵染后的组培苗(与黄萎菌共培养)和体内杀菌苗(温室内生长3周后)的植株生长和病害发展进行了测定。在体外细菌化植株和未细菌化植株(对照)之间,无论是对病害的抑制还是对植株的生长都有显著差异。体外细菌化的保护程度取决于大丽弧菌的接种量,最低(103个孢子·m L-1)和最高(106个孢子·m L-1)的保护效果最好和最差。相比之下,体内细菌化的番茄与未细菌化的对照植物相比,并没有表现出促进植物生长的作用。当挑战Vertic时..。
The potential utilization of a plant growth promoting rhizobacterium, Pseudomonas sp. strain PsJN, to enhance the resistance of tomato transplants to verticillium wilt was investigated. Plant growth and disease development were tested on the disease-susceptible cultivar Bonny Best after Verticillium dahliae infection of tissue culture plantlets bacterized in vitro (by co-culturing with the bacterium) and seedlings bacterized in vivo (after 3 weeks growth in the greenhouse). Significant differences in both disease suppression and plant growth were obtained between in vitro bacterized and nonbacterized (control) plants. The degree of protection afforded by in vitro bacterization depended on the inoculum density of V. dahliae; the best and worst protection occurred at the lowest (103 conidia ·mL-1) and highest (106 conidia ·mL-1) levels, respectively. In contrast, the in vivo bacterized tomatoes did not show plant growth promotion when compared to the nonbacterized control plants. When challenged with Vertic...