QUANTITATIVE-ANALYSIS OF EXTRACELLULAR-SPACE USING THE METHOD OF TMA+ IONTOPHORESIS AND THE ISSUE OF TMA+ UPTAKE
QUANTITATIVE-ANALYSIS OF EXTRACELLULAR-SPACE USING THE METHOD OF TMA+ IONTOPHORESIS AND THE ISSUE OF TMA+ UPTAKE
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DOI：
10.1139/y92-278
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发表时间：
1992-01-01
影响因子：
2.1
通讯作者：
NICHOLSON, C
中科院分区：
文献类型：
--
作者：
NICHOLSON, C
详细介绍了四甲基铵（TMA+）法测量脑细胞外间隙体积分数和迂曲度的方法。离子电渗或压力显微注射后细胞外空间中TMA+的时空分布由合适的方程描述，并用图表说明。通过将方程拟合到从离子选择性微量移液管测量获得的浓度与时间的数据，可以测量体积分数和弯曲度。此外，TMA+的浓度依赖性摄取可根据给定方程估计。论文的最后一部分通过这种机制推导出细胞外空间TMA+损失的简单数值估计。
The tetramethylammonium (TMA+) method for measuring the volume fraction and tortuosity of brain extracellular space is presented in detail. The temporal and spatial distribution of TMA+ in the extracellular space following iontophoresis or pressure microinjection is described by suitable equations and illustrated with graphs. By fitting the equations to the concentration versus time data obtained from measurements with ion-selective micropipettes, the volume fraction and tortuosity can be measured. In addition, the concentration-dependent uptake of TMA+ can be estimated from the given equations. The final section of the paper derives simple numerical estimates of TMA+ loss from the extracellular space by this mechanism.