Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment
Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment
复制标题
联合预处理后分批补料同步糖化发酵高干物质玉米芯生产乙醇
DOI：
10.1016/j.biortech.2009.11.010
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发表时间：
2010-07-01
影响因子：
11.4
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Zhang, Mingjia;Wang, Fang;He, Zhimin
为了从纤维素中提取高浓度乙醇，对玉米芯进行酸碱处理，去除非纤维素成分，然后进行同步糖化发酵(SSF)。采用间歇固态发酵工艺，以19%的干物质(DM)可获得高达69.2g/L的乙醇浓度，总酒精得率为81.2%。还研究了高固相浓度下的进料间歇过程。新鲜基质用稀硫酸-氢氧化钠处理，然后在第一个24 h内按不同的量添加，最终得到干物质含量为25%(w/v)的产品。在纤维素量为22.8FPU/g葡聚糖、干酵母(酿酒酵母)装液量为5g/L、每4h补料一次的固态发酵条件下，发酵96h后酒精浓度最高，达84.7g/g，总酒精得率为79%。(C)2009爱思唯尔有限公司。保留所有权利。
To obtain high concentration of ethanol from cellulose, corncob was pretreated with acid and alkali to remove non-cellulose components, and then subjected to simultaneous saccharification and fermentation (SSF). An ethanol concentration as high as 69.2 g/L was achieved with 19% dry matter (DM) using batch SSF, resulting in an 81.2% overall ethanol yield. A fed-batch process using a high solid concentration was also investigated. Fresh substrate was pretreated with dilute sulfuric acid-sodium hydroxide, and then added at different amounts during the first 24 h, to yield a final dry matter content of 25% (w/v). SSF conditions with cellulose loading of 22.8 FPU/g glucan, dry yeast (Saccharomyces cerevisiae) loading of 5 g/L and substrate supplementation every 4 h yielded the highest ethanol concentration of 84.7 g/L after 96 h. This corresponded to a 79% overall ethanol yield. (C) 2009 Elsevier Ltd. All rights reserved.