Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE
Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE
复制标题
DOI：
10.1128/jb.181.22.7043-7051.1999
复制
发表时间：
1999-11-01
影响因子：
3.2
通讯作者：
Driks, A
中科院分区：
文献类型：
--
作者：
Bauer, T;Little, S;Driks, A
The Bacillus subtilis spore is encased in a resilient, multilayered proteinaceous shell, called the coat, that protects it from the environment. A 181-amino-acid coat protein called CotE assembles into the coat early in spore formation and plays a morphogenetic role in the assembly of the coat's outer layer. We have used a series of mutant alleles of cotE to identify regions involved in outer coat protein assembly. We found that the insertion of a 10-amino-acid epitope, between amino acids 178 and 179 of CotE, reduced or prevented the assembly of several spore coat proteins, including, most likely, CotG and CotB. The removal of 9 or 23 of the C-terminal-most amino acids resulted in an unusually thin outer coat from which a larger set of spore proteins was missing. In contrast, the removal of 37 amino acids from the C terminus, as well as other alterations between amino acids 4 and 160, resulted in the absence of a detectable outer coat but did not prevent localization of CotE to the forespore. These results indicate that changes in the C-terminal 23 amino acids of CotE and in the remainder of the protein have different consequences for outer coat protein assembly.
