An experimentally derived database of candidate ras-interacting proteins
An experimentally derived database of candidate ras-interacting proteins
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DOI：
10.1021/pr060630l
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Ginsberg, Mark H.
中科院分区：
文献类型：
--
作者：
Goldfinger, Lawrence E.;Ptak, Celeste;Ginsberg, Mark H.
我们使用 TAP 标签方法来鉴定相关 Ras 家族 GTPases 的候选结合蛋白：H-Ras、R-Ras 和 Rap1A。从小鼠成纤维细胞中分离出蛋白质复合物，并通过纳流 HPLC 和串联质谱法的组合鉴定成分蛋白质。发现 H-Ras 与包括 talin-1 在内的多种细胞骨架蛋白相关。 R-Ras 和 Rap1A 各自与各种信号分子相关，其中许多与膜相关。由此，我们建立了第一个哺乳动物细胞中潜在Ras相互作用子数据库。 关键词：蛋白质组学中心点质谱中心点GTPases中心点Ras
We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases: H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells.Keywords: proteomics center dot mass spectrometry center dot GTPases center dot Ras