The Diagnostic Significance of PDGF, EphA7, CCR5, and CCL5 Levels in Colorectal Cancer
The Diagnostic Significance of PDGF, EphA7, CCR5, and CCL5 Levels in Colorectal Cancer
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DOI：
10.3390/biom9090464
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发表时间：
2019-09
期刊：
影响因子：
5.5
通讯作者：
M. Üçüncü;M. Serilmez;M. Sari;S. Bademler;S. Karabulut
中科院分区：
文献类型：
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作者：
M. Üçüncü;M. Serilmez;M. Sari;S. Bademler;S. Karabulut
本研究比较了结直肠癌患者和健康对照组C-C趋化因子受体5(CCR5)、C-C基序趋化因子配体5(CCL5)、血小板衍生生长因子(PDGF)和肾上腺素A7(EphA7)的水平，以探讨这些潜在的生物标志物在结直肠癌早期诊断中的意义和实用性。这项研究包括70名结直肠癌患者和40名健康人。用双抗体夹心法测定血样中CCR5、CCL5、PDGF和EphA7水平。结直肠癌患者的血小板衍生生长因子-BB、EphA7、CCR5和CCL5水平均显著高于对照组(P<；各为0.001)。我们的Logistic回归分析(曲线下面积为0.958)支持这一观点，即PDGFBB、EphA7和CCL5是诊断结肠癌的潜在生物标志物。其敏感性、特异性、阳性预测值和阴性预测值分别为87.9%、87.5%、92.1%和81.4%。据我们所知，这是第一次研究结直肠癌与CCL5、CCR5、PDGF和EphA7四个生物标记物之间的关系。患者组上述各项指标均显著高于健康对照组，提示它们可用于结直肠癌的早期诊断。
In this study, we compared the levels of C-C chemokine receptor type 5 (CCR5), C-C motif chemokine ligand 5 (CCL5), platelet-derived growth factor (PDGF), and EphrinA7 (EphA7) in patients with colorectal carcinoma and healthy controls in order to investigate the significance and usability of these potential biomarkers in early diagnosis of colorectal cancer. The study included 70 colorectal carcinoma patients and 40 healthy individuals. The CCR5, CCL5, PDGF, and EphA7 levels were measured using ELISA in blood samples. PDGF-BB, EphA7, CCR5, and CCL5 levels of the patients with colorectal carcinoma were significantly higher compared to the control group (p < 0.001 for each comparison). Our logistic regression analysis (the area under the curve was 0.958) supports the notion that PDGF-BB, EphA7, and CCL5 are potential biomarkers for the diagnosis of colon cancer. The sensitivity, specificity, and positive and negative predictive values were found to be 87.9%, 87.5%, 92.1%, and 81.4%, respectively. To our knowledge, this is the first study that investigates the relationship between colorectal carcinoma and the four biomarkers CCL5, CCR5, PDGF, and EphA7. The significantly elevated levels of all these parameters in the patient group compared to the healthy controls indicate that they can be used for the early diagnosis of colorectal carcinoma.