PREPARATIVE INVITRO MESSENGER-RNA SYNTHESIS USING SP6 AND T7 RNA-POLYMERASES
PREPARATIVE INVITRO MESSENGER-RNA SYNTHESIS USING SP6 AND T7 RNA-POLYMERASES
复制标题
DOI：
10.1016/0003-2697(91)90318-n
复制
发表时间：
1991-06-01
影响因子：
2.9
通讯作者：
ZOZULYA, SA
中科院分区：
文献类型：
--
作者：
GUREVICH, VV;POKROVSKAYA, ID;ZOZULYA, SA
提出了一种制备性体外mRNA合成的方法。该方法使得每分子线性化DNA模板产生数百个RNA拷贝成为可能。该方案适用于不同长度（200至3000个碱基）的RNA的合成，并且可以使用SP 6和T7 RNA聚合酶。转录可以扩大到至少6-12 ml，产生4.5-18 mg的同质生物活性mRNA。
A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6–12 ml, yielding 4.5–18 mg of homogeneous biologically active mRNA.