The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase.
The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase.
复制标题
编码大鼠前列环素合酶的 mRNA 的区域分布和细胞定位。
DOI：
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发表时间：
1997
影响因子：
6.6
通讯作者：
Tadashi Tanabe
中科院分区：
文献类型：
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作者：
Yoshinori Tone;Hiroyasu Inoue;Shuntaro Hara;C. Yokoyama;T. Hatae;Hiroji Oida;S. Narumiya;R. Shigemoto;Susumu Yukawa;Tadashi Tanabe
克隆的大鼠前列环素合成酶基因包含一个1503个碱基的开放阅读框，编码501个氨基酸的蛋白质，与人类前列环素合成酶的同源性为84.0%。RNA印迹分析表明，大鼠前列环素合成酶mRNA作为一个2.1kb的单一物种，在主动脉和子宫中大量表达。在胃、肺、心脏、睾丸、肝脏和骨骼肌中也观察到高水平的表达。大脑和肾脏中也有少量但显著的表达。组织切片原位杂交分析前列环素合成酶mRNA的区域分布和细胞定位。该信使核糖核酸定位于动脉、支气管和子宫的平滑肌细胞以及生精小管周围的纤维膜细胞中，这些细胞的特征是平滑肌细胞。除平滑肌细胞外，心脏成纤维细胞、肺实质细胞、肾内髓质小管和间质细胞中也有信号。
The cloned cDNA for rat prostacyclin synthase was found to contain a 1503-bp open reading frame which encoded a 501-amino acid protein sharing 84.0% identity with the human enzyme. RNA blot analysis revealed that the rat prostacyclin synthase mRNA, as a single species of 2.1 kb, is expressed abundantly in the aorta and uterus. High levels of expression were also observed in the stomach, lung, heart, testis, liver, and skeletal muscle. Low but significant expression was also seen in the brain and kidney. Furthermore, the regional distribution and cellular localization of prostacyclin synthase mRNA were examined by in situ hybridization analysis of rat tissue sections. The definitive signals for the mRNA were localized in smooth muscle cells of the arteries, bronchi and uterus, and in the cells of the fibrous tunic surrounding the seminiferous tubules, which are characterized as smooth muscle cells. Besides smooth muscle cells, signals were also detected in the fibroblasts of the heart myocardium, lung parenchyma cells and kidney inner medulla tubules and interstitial cells.