Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography.
Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography.
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通过高效液相色谱分析还原糖的发色腙。
DOI：
10.1016/0003-2697(87)90416-7
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发表时间：
1987
影响因子：
2.9
通讯作者：
H. Kamiya
中科院分区：
文献类型：
--
作者：
K. Muramoto;R. Goto;H. Kamiya
以4′-N，N-二甲氨基-4-偶氮苯磺酰氯为原料，与肼反应制得发色酰肼4 ′-N，N-二甲氨基-4-偶氮苯磺酰肼（DABS-酰肼）。还原糖在50°C下用DABS-酰肼衍生化120 min。发色团腙通过反相HPLC等度分离，使用短柱（4.6 × 50 mm）和0.08 m乙酸-丙酮作为洗脱剂，无需样品预处理，并在皮摩尔水平下定量。将该方法应用于5 μg糖蛋白的糖分析。糖的丹酰肼衍生物也通过该HPLC系统分离。
A chromophoric hydrazide, 4′-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4′-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50°C for 120 min. The chromophoric hydrazones were separated by reversed-phase HPLC isocratically using a short column (4.6 × 50 mm) and 0.08 m acetic acid-acetone as an eluant with no sample pretreatment and were quantitated at the picomole level. This method was applied to the sugar analysis of 5 μg of glycoproteins. Dansyl hydrazide derivatives of sugars were also separated by this HPLC system.
影响因子：
2.9
作者：
WANG, WT;LEDONNE, NC;SWEELEY, CC
通讯作者：
SWEELEY, CC