Delivery of epitopes by the Salmonella type III secretion system for vaccine development
Delivery of epitopes by the Salmonella type III secretion system for vaccine development
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DOI：
10.1126/science.281.5376.565
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发表时间：
1998-07-24
期刊：
影响因子：
56.9
通讯作者：
Donis, RO
中科院分区：
文献类型：
--
作者：
Rüssmann, H;Shams, H;Donis, RO
鼠伤寒沙门氏菌的无毒株被认为是抗原递送载体。在宿主的细胞内阶段，鼠伤寒沙门氏菌驻留在膜结合的隔室中，并且不是 I 类限制性免疫反应的有效诱导剂。通过使用鼠伤寒沙门氏菌的 III 型蛋白分泌系统，病毒表位成功递送至宿主细胞胞质。这导致了 I 类限制性免疫反应，保护接种疫苗的动物免受致命感染。这种方法可以有效地利用鼠伤寒沙门氏菌作为抗原递送系统来控制需要这种类型的免疫反应来保护的病原体的感染。
Avirulent strains of Salmonella typhimurium are being considered as antigen delivery vectors. During its intracellular stage in the host, S. typhimurium resides within a membrane-bound compartment and is not an efficient inducer of class I-restricted immune responses. Viral epitopes were successfully delivered to the host-cell cytosol by using the type III protein secretion system of S. typhimurium. This resulted in class I-restricted immune responses that protected vaccinated animals against lethal infection. This approach may allow the efficient use of S. typhimurium as an antigen delivery system to control infections by pathogens that require this type of immune response for protection.