Improved lignin degradation through distinct microbial community in subsurface sediments of one eutrophic lake
Improved lignin degradation through distinct microbial community in subsurface sediments of one eutrophic lake
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通过富营养化湖泊地下沉积物中独特的微生物群落改善木质素降解
DOI：
10.1016/j.renene.2019.01.121
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发表时间：
2019-08-01
期刊：
影响因子：
8.7
通讯作者：
Jiang, He-Long
中科院分区：
文献类型：
--
作者：
Song, Na;Xu, Huacheng;Jiang, He-Long
木质纤维素是一种很有前途的可持续替代燃料，其分解对沉积物有机质的形成至关重要。木质纤维素的木质素限制了酶进入纤维素的途径，减缓了纤维素的分解。因此，开发高效的微生物群落来分解木质素具有重要意义。本研究通过715天的原位实验，研究了富营养化湖泊不同深度（38 cm）沉积物中芦苇（Phragmites australis）碎屑的降解过程。随着深度的增加，凋落物质量降解呈明显的下降趋势，但表层沉积物中木质素的降解效率显著高于表层沉积物（p < 0.05）。焦磷酸测序结果表明，沉积物中含有特定的厌氧细菌（硫菌属、梭菌属和密螺旋体属）和厌氧真菌（壶菌门和罗氏菌门）。这些厌氧微生物很好地适应了难降解有机物的降解，并导致了地下沉积物中木质素生物降解活性的增加。这项工作有助于提高我们对木质素生物降解的认识，并为有效的生物燃料生产开发潜在的生物技术。(C) 2019 Elsevier Ltd.版权所有。
Lignocellulose is a promising sustainable alternative fuel and its decomposition is critical for the formation of sediment organic matter. The lignin of lignocellulose limits the access of enzymes to cellulose and slows down the decomposition. Therefore, the development of efficient microbial consortia to deconstruct lignin is of great interest. In this study, a 715-day in situ experiment was performed to investigate the degradation process of macrophyte Phragmites australis debris at different depths of sediments (up to 38 cm) in a eutrophic lake. Although litter mass degradation showed an obvious decreasing tendency as the depth increased, the efficiency of lignin degradation in subsurface sediments was significance higher than that in surface sediments (p < 0.05). Pyrosequencing analysis showed that the subsurface sediments contained the specific anaerobic bacteria (genus Sulfuricurvum, Clostridium and Treponema) and anaerobic fungi (phylum Chytridiomycota and Rozellomycota). These anaerobic microbes well adapted to the degradation of recalcitrant organic matter, and led to the increasing activity of lignin biodegradation in subsurface sediments. This work could help improve our understanding of lignin biodegradation and develop a potential biotechnology for efficient biofuel production. (C) 2019 Elsevier Ltd. All rights reserved.