Mstu1, an APSES Transcription Factor, Is Required for Appressorium-Mediated Infection in Magnaporthe grisea
Mstu1, an APSES Transcription Factor, Is Required for Appressorium-Mediated Infection in Magnaporthe grisea
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DOI：
10.1271/bbb.90146
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发表时间：
2009-08-01
影响因子：
1.6
通讯作者：
Hayashi, Nagao
中科院分区：
文献类型：
--
作者：
Nishimura, Marie;Fukada, Junji;Hayashi, Nagao
APSES 蛋白家族包括子囊菌形态过程的重要转录调节因子。我们在稻瘟病菌中发现了 APSES 蛋白 Mstu1 的缺失突变体，该突变体表现出分生孢子形成和菌丝生长减少。 Mstu1 形成了许多与野生型相当的附着胞，尽管附着胞的形成被延迟。在稻瘟病菌中，分生孢子糖原和脂滴快速转移到初期的附壁是产生附壁膨压所必需的，真菌利用附壁膨压来穿透植物角质层。 mstu1 的附着膨压较低，并且该突变体缺乏附着胞介导的水稻叶片入侵。 mstu1 中分生孢子糖原和脂滴的转移显着延迟，并且观察到这些分生孢子储备的降解随之延迟。我们的结果表明，Mstu1 是附着细胞介导的感染所必需的，因为它参与脂质和糖原的动员。
The APSES protein family includes important transcriptional regulators of morphological processes in ascomycetes. We identified a deletion mutant of the APSES protein Mstu1 in Magnaporthe grisea that showed reduced conidiation and mycelial growth. Mstu1 formed a number of appressoria comparable to the wild type, although appressorium formation was delayed. In M. grisea, rapid transfer of conidial glycogen and lipid droplets to incipient appressoria is required for appressorial turgor generation, which the fungus uses to penetrate plant cuticles. Appressorial turgor was low in mstu1 and the mutant was deficient in appressorium-mediated invasion of rice leaves. The transfer of conidial glycogen and lipid droplets was remarkably delayed in mstu1, and a consequent delay in degradation of these conidial reserves was observed. Our results indicate that Mstu1 is required for appressorium-mediated infection due to its involvement in the mobilization of lipids and glycogen.