GEL FILTRATION OF PROTEINS, WITH PARTICULAR REFERENCE TO THE GLYCOPROTEIN, LUTEINIZING HORMONE.
GEL FILTRATION OF PROTEINS, WITH PARTICULAR REFERENCE TO THE GLYCOPROTEIN, LUTEINIZING HORMONE.
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蛋白质的凝胶过滤，特别是糖蛋白、黄体生成素。
DOI：
10.1016/0003-2697(65)90094-1
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发表时间：
1965
影响因子：
2.9
通讯作者：
M. S. Arnott
中科院分区：
文献类型：
--
作者：
D. N. Ward;M. S. Arnott
结果表明，在pH为1.3时，促黄体生成素（LH）在pH为1.3和pH为7.5时，与一系列简单蛋白和另外两种糖蛋白在Sephadex G-75上的凝胶过滤行为相比，其物理尺寸发生了显著变化。包括pH 7.5时Bio-Gel P-100的比较数据。凝胶过滤柱的校准不适用于糖蛋白分子量的估计，因为可用于参考校准的糖蛋白数量有限。
Results are reported which show that luteinizing hormone (LH) at pH 1.3 undergoes a marked change in its physical dimensions as studied by comparison with the gel filtration behavior of a series of simple proteins and two other glycoproteins on Sephadex G-75 at pH 1.3 and pH 7.5. Comparative data for Bio-Gel P-100 at pH 7.5 is included. The calibration of the gel filtration columns is not suitable for estimation of molecular weight of glycoproteins with the limited number of glycoproteins available for reference calibration.