Microwave irradiation induces neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
Microwave irradiation induces neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
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DOI：
10.1016/j.neulet.2007.12.002
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发表时间：
2008-02-13
影响因子：
2.5
通讯作者：
Kano, Yoshio
中科院分区：
文献类型：
--
作者：
Inoue, Shigeki;Motoda, Hirotoshi;Kano, Yoshio
随着移动的电话通信的日益普及，人们开始关注微波辐射可能对健康造成的危害。本文研究了微波辐射对药物敏感的PC12细胞株（PC12m3）的损伤和诱导分化作用。这些细胞表现出对各种刺激物的神经突生长的增强。由2.45 GHz（200 W）的微波照射诱导的神经突生长的频率比未照射的对照细胞的频率高约10倍。用p38 MAPK特异性抑制剂SB 203580处理PC12m3细胞，可明显抑制微波辐射诱导的神经突起生长。SB203580还抑制了微波辐射诱导的转录因子CREB的激活。45 ℃热休克处理对PC 12m3细胞有较强的毒性作用，而微波处理对PC 12m3细胞无毒性作用。这些结果表明，p38 MAPK是负责PC 12 m3细胞的存活，并可能通过CREB信号通路时，受到微波照射诱导神经突起生长。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
The increasing use of mobile phone communication has raised concerns about possible health hazard effects of microwave irradiation. We investigated damage and differentiation caused by microwave irradiation on drug-hypersensitive PC12 cell line (PC12m3). These cells showed enhancement of neurite outgrowth to various stimulants. The frequency of neurite outgrowth induced by 2.45 GHz (200 W) of microwave irradiation was approximately 10-fold greater than that of non-irradiated control cells. Incubation of PC12m3 cells with SB203580, a specific inhibitor of p38 MAPK, resulted in marked inhibition of the microwave radiation-induced neurite outgrowth. Also, activation of the transcription factor CREB induced by microwave irradiation was inhibited by SB203580. Heat shock treatment at 45 degrees C had a strong toxic effect on PC 12m3 cells, whereas microwave treatment had no toxic effect on PC12m3 cells. These findings indicate that p38 MAPK is responsible for the survival of PC 12m3 cells and might induce neurite outgrowth via a CREB signaling pathway when subjected to microwave irradiation. (c) 2007 Elsevier Ireland Ltd. All rights reserved.