Gene expression profiles of normal human fibroblasts after exposure to ionizing radiation: A comparative study of low and high doses
Gene expression profiles of normal human fibroblasts after exposure to ionizing radiation: A comparative study of low and high doses
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DOI：
10.1667/rr3354
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Chen, DJ
中科院分区：
文献类型：
--
作者：
Ding, LH;Shingyoji, M;Chen, DJ
对电离辐射的几种细胞反应，如适应性反应或旁观者效应，表明低剂量辐射可能具有区别于高剂量辐射的特征。累积的证据还表明，低剂量和高剂量电离辐射的生物效应不是线性分布的。我们已经调查了，第一次，全球基因表达的变化诱导的电离辐射剂量低至2 c戈伊，并比较这在4戈伊的表达变化。我们应用基因微阵列分析G（1）阻滞的正常人皮肤成纤维细胞受到X射线照射。我们的数据表明，定性和定量差异之间存在的基因表达谱诱导的2 c戈伊和4戈伊。对低剂量辐射作出反应的主要功能群是那些参与细胞间信号传递、信号传导、发育和DNA损伤反应的功能群。在高剂量下，应答基因参与细胞凋亡和细胞增殖。有趣的是，一些基因，如细胞骨架成分ANLN和KRT 15以及细胞-细胞信号传导基因GRAP 2和GPR 51，被发现对低剂量辐射有反应，但对高剂量辐射没有反应。低剂量辐射特别激活的途径也很明显。这些基因表达变化的定量和定性差异可能有助于解释电离辐射生物效应从低剂量到高剂量的非线性相关性。(c)2005年，辐射研究协会。
Several types of cellular responses to ionizing radiation, such as the adaptive response or the bystander effect, suggest that low-dose radiation may possess characteristics that distinguish it from its high-dose counterpart. Accumulated evidence also implies that the biological effects of low-dose and high-dose ionizing radiation are not linearly distributed. We have investigated, for the first time, global gene expression changes induced by ionizing radiation at doses as low as 2 cGy and have compared this to expression changes at 4 Gy. We applied cDNA microarray analyses to G(1)-arrested normal human skin fibroblasts subjected to X irradiation. Our data suggest that both qualitative and quantitative differences exist between gene expression profiles induced by 2 cGy and 4 Gy. The predominant functional groups responding to low-dose radiation are those involved in cell-cell signaling, signal transduction, development and DNA damage responses. At high dose, the responding genes are involved in apoptosis and cell proliferation. Interestingly, several genes, such as cytoskeleton components ANLN and KRT15 and cell-cell signaling genes GRAP2 and GPR51, were found to respond to low-dose radiation but not to high-dose radiation. Pathways that are specifically activated by low-dose radiation were also evident. These quantitative and qualitative differences in gene expression changes may help explain the non-linear correlation of biological effects of ionizing radiation from low dose to high dose. (c) 2005 by Radiation Research Society.