Viridibacillus soli sp. nov., isolated fromforest soil inAilaoshan National Nature Reserve
Viridibacillus soli sp. nov., isolated fromforest soil inAilaoshan National Nature Reserve
复制标题
土壤绿色芽孢杆菌 sp.
DOI：
10.1007/s00203-021-02676-x
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发表时间：
2021
影响因子：
2.8
通讯作者：
Shu‑Kun Tang
中科院分区：
文献类型：
--
作者：
Yan Xu;Jiang‑Yuan Zhao;Yu Geng;Hui‑Ren Yuan;Le‑Le Li;Meng‑Liang Wen;Ming‑Gang Li;Shu‑Kun Tang
从中国云南省新平县玉溪市哀牢山国家级自然保护区采集的森林土壤样品中分离出一株革兰氏染色阳性、杆状、近末端内生孢子形成细菌，命名为菌株YIM B01967T。菌株YIM B01967T与Viridibacillus arvi (99.1%)和Viridibacillus arenosi (98.9%)的16S rRNA基因序列相似性最高。根据系统发育和16S rRNA基因序列结果，菌株YIM B01967T属于Viridibacillus属。 YIM B01967T 的生长在 15–35 °C（最佳，28 °C）、pH 7.0–9.0（最佳，pH 7.5）和 0–2% (w/v) NaCl（最佳 2% NaCl）存在下观察到。细胞壁糖包括核糖、葡萄糖、阿拉伯糖、半乳糖和甘露糖。醌系统由主要化合物MK-8和适量的MK-7组成。主要脂肪酸(> 10%)包括iso-C15:0、anteiso-C15:0、C16:1ω10c。主要的极性脂质包括 DPG、PME。细胞壁肽聚糖最有可能是具有 1-Lys-d-Asp 肽间桥的 A4α 型。菌株YIM B01967T的基因组DNA G + C含量为36.3mol%。根据基因组草图序列，菌株 YIM B01967T 与 Viridibacillus arviDSM 16317T、Viridibacillus arenosiDSM 16319T 之间的 ANI 和数字 DNA-DNA 杂交 (dDDH) 值分别为 61.0% 和 32.1%、60.0% 和 33.1%。结果支持菌株YIM B01967T代表Viridibacillus属的新种的结论，其名称为Viridibacillus solisp。十一月，提出。模式菌株为YIM B01967T(= KCTC 43249T= CGMCC 1.18436T)。
A Gram stain-positive, rod-shaped, and subterminal endospore-forming bacterium, designated strain YIM B01967T, was isolated from a forest soil sample collected in Ailaoshan National Nature Reserve, Yuxi City, Xinpin county, Yunnan province, China. Strain YIM B01967Tshowed the highest 16S rRNA gene sequence similarity withViridibacillus arvi(99.1%) andViridibacillus arenosi(98.9%). Based on the phylogenetic and 16S rRNA gene sequence results, strain YIM B01967Twas affiliated to the genusViridibacillus. The growth of YIM B01967Twas observed at 15–35 °C (optimum, 28 °C), pH 7.0–9.0 (optimum, pH 7.5) and in the presence of 0–2% (w/v) NaCl (optimum in 2% NaCl). The cell wall sugars include ribose, glucose, arabinose, galactose, and mannose. The quinone system consisted of the major compound MK-8 and moderate amounts of MK-7. The major fatty acids (> 10%) included iso-C15:0, anteiso-C15:0, C16:1ω10c. The major polar lipids profile included DPG, PME. The cell wall peptidoglycan was most likely of the type A4αwith anl-Lys-d-Asp interpeptide bridge. The genomic DNA G + C content of strain YIM B01967Twas 36.3 mol%. The ANI and digital DNA–DNA hybridization (dDDH) values between strain YIM B01967TandViridibacillus arviDSM 16317T,Viridibacillus arenosiDSM 16319Twere 61.0% and 32.1%, 60.0% and 33.1% based on the draft genome sequence. The results support the conclusion that strain YIM B01967Trepresents a novel species of the genusViridibacillus, for which the nameViridibacillus solisp. nov., is proposed. The type strain is YIM B01967T(= KCTC 43249T= CGMCC 1.18436T).