White cell and platelet content affects the release of bioactive factors in different blood-derived scaffolds
White cell and platelet content affects the release of bioactive factors in different blood-derived scaffolds
复制标题
DOI：
10.1080/09537104.2017.1319046
复制
发表时间：
2018-01-01
期刊：
影响因子：
3.3
通讯作者：
Riccitiello, F.
中科院分区：
文献类型：
--
作者：
Cabaro, S.;D'Esposito, V.;Riccitiello, F.
血小板衍生因子是一种生物材料，可以加速口腔、颌面部和其他几种应用的愈合过程。浓缩血小板释放特定因子对于取得成功结果至关重要。在这里，我们发现富含血小板的纤维蛋白（PRF）凝块是白细胞的有益来源，可能直接影响趋化因子和生长因子的释放。与标准白细胞 PRF (L-PRF) 相比，实验性低力改良程序 [定义为高级 PRF (A-PRF)] 捕获相同含量的活白细胞，释放相似量的炎症细胞因子，但分泌的 Eotaxin、CCL5、血小板衍生生长因子 (PDGF) 和血管内皮生长因子水平高出 3、1.6、3 和 1.2 倍(VEGF)，分别。无白细胞支架，例如富含生长因子 (PRGF) 的血浆，仅释放血小板特异性因子，特别是生长因子最丰富的 F3 级分，与 FI 和 F2 级分相比，分泌更高量的 CCL5 和 PDGF。总之，不同的程序和白细胞含量会影响血小板衍生物中细胞因子、趋化因子和生长因子的释放，这可能对不同的临床环境有所帮助。
Platelet-derived factors are biomaterials that might accelerate healing process in oral, maxillofacial, and several other applications. Release of specific factors by platelet concentrates is critical to achieving a successful outcome. Here, we have shown that platelet-rich fibrin (PRF) clots were beneficial sources of leukocytes, which may directly affect the release of chemokines and growth factors. When compared with the standard leukocyte-PRF (L-PRF), the experimental low-force modified procedure [defined as advanced-PRF (A-PRF)] entrapped the same content of viable leukocytes, released a similar amount of inflammatory cytokines, but secreted 3-, 1.6-, 3-, and 1.2fold higher levels of Eotaxin, CCL5, platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF), respectively. A leukocyte-free scaffold, such as plasma rich in growth factors (PRGF), released only platelet-specific factors and, in particular, the F3 fraction, the richest in growth factors, secreted higher amount of CCL5 and PDGF compared to FI and F2 fractions. In conclusion, different procedures and leukocyte content affect cytokine, chemokines, and growth factor release from platelet derivatives, which may be helpful in different clinical settings.