Temporal Organization of GABAergic Interneurons in the Intermediate CA1 Hippocampus During Network Oscillations
Temporal Organization of GABAergic Interneurons in the Intermediate CA1 Hippocampus During Network Oscillations
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DOI：
10.1093/cercor/bht316
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发表时间：
2015-05-01
期刊：
影响因子：
3.7
通讯作者：
Klausberger, Thomas
中科院分区：
文献类型：
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作者：
Forro, Thomas;Valenti, Ornella;Klausberger, Thomas
旅行θ振荡和尖锐的波相关的波纹（SWR）提供的时间结构，在CA 1海马神经活动。产生节律的GABA能中间神经元对跨隔颞CA 1轴的网络计时的贡献仍然未知。我们记录了尖峰时间的识别parvalbumin（PV）表达篮，轴-轴，定向-腔隙分子（O-LM）中间神经元，和锥体细胞在中间CA 1（iCA 1）的麻醉大鼠在同时检测到的网络振荡在iCA 1和背侧CA 1（dCA 1）。不同的中间神经元类型的耦合差异SWR，和大多数的iCA 1 SWR事件同时发生与dCA 1 SWR事件。相比之下，iCA 1 θ振荡相对于dCA 1 θ振荡在时间上移位。在θ周期中，iCA 1轴-轴细胞的最高发射其次是PV表达的篮状细胞，随后是O-LM和锥体细胞，类似于先前报道的dCA 1细胞类型的发射序列。然而，我们观察到，这种细胞类型的时间组织在dCA 1和iCA 1之间发生了时间转移，同时θ振荡也发生了相应的转移。我们发现，GABA能活动可以同步在SWR，但在θ振荡期间从dCA 1到iCA 1的时间转移，突出了灵活的抑制控制整个大脑结构的兴奋性活动。
Travelling theta oscillations and sharp wave-associated ripples (SWRs) provide temporal structures to neural activity in the CA1 hippocampus. The contribution of rhythm-generating GABAergic interneurons to network timing across the septotemporal CA1 axis remains unknown. We recorded the spike-timing of identified parvalbumin (PV)-expressing basket, axo-axonic, oriens-lacunosum moleculare (O-LM) interneurons, and pyramidal cells in the intermediate CA1 (iCA1) of anesthetized rats in relation to simultaneously detected network oscillations in iCA1 and dorsal CA1 (dCA1). Distinct interneuron types were coupled differentially to SWR, and the majority of iCA1 SWR events occurred simultaneously with dCA1 SWR events. In contrast, iCA1 theta oscillations were shifted in time relative to dCA1 theta oscillations. During theta cycles, the highest firing of iCA1 axo-axonic cells was followed by PV-expressing basket cells and subsequently by O-LM together with pyramidal cells, similar to the firing sequence of dCA1 cell types reported previously. However, we observed that this temporal organization of cell types is shifted in time between dCA1 and iCA1, together with the respective shift in theta oscillations. We show that GABAergic activity can be synchronized during SWR but is shifted in time from dCA1 to iCA1 during theta oscillations, highlighting the flexible inhibitory control of excitatory activity across a brain structure.