Protection afforded against aerosol challenge by systemic immunisation with inactivated Francisella tularensis live vaccine strain (LVS)
Protection afforded against aerosol challenge by systemic immunisation with inactivated Francisella tularensis live vaccine strain (LVS)
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DOI：
10.1016/j.micpath.2007.08.009
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发表时间：
2008-02-01
影响因子：
3.8
通讯作者：
Titball, R. W.
中科院分区：
文献类型：
--
作者：
Eyles, J. E.;Hartley, M. G.;Titball, R. W.
用土拉热弗朗西丝氏菌灭活活疫苗株（LVS）免疫BALB/c小鼠，观察其对土拉热弗朗西丝氏菌强毒株气溶胶攻击的保护水平。土拉菌已确定。肌内（IM）注射灭活的LVS与氢氧化铝为基础的澄清剂刺激的IgGI偏置的LVS特异性抗体反应，并提供了对气溶胶挑战与亚种holarctica（菌株HN 63）没有保护作用。相反，IM注射灭活的LVS佐剂与预先形成的免疫刺激复合物（ISCOMS）与免疫刺激性CpG寡核苷酸混合提供了强大的保护，对气溶胶引发的感染HN 63。然而，尽管相对于未经处理的对照组，至死亡时间显著延长，但用最有效的疫苗制剂免疫的大多数小鼠不能抵抗土拉菌亚种（菌株Schu S4）的低剂量气溶胶攻击。这些数据表明，非肠道给药的非活疫苗可用于有效的免疫接种，对抗全北极亚种的气溶胶攻击，尽管不是高毒力的F。土拉热。皇冠版权所有（C）2007由爱思唯尔有限公司出版。保留所有权利。
BALB/c mice were immunised with inactivated Francisella tularensis live vaccine strain (LVS) and the level of protection afforded against aerosol challenge with virulent strains of F. tularensis ascertained. Intramuscular (IM) injection of inactivated LVS with an aluminium-hydroxide-based adjuvant-stimulated IgGI-biased LVS-specific antibody responses and afforded no protection against aerosol challenge with subspecies holarctica (strain HN63). Conversely, IM injection of inactivated LVS adjuvanted with preformed immune-stimulating complexes (ISCOMS) admixed with immumostimulatory CpG oligonucleotides afforded robust protection against aerosol-initiated infection with HN63. However, despite a significantly extended time-to-death relative to naYve controls, the majority of mice immunised with the most potent vaccine formulation were not protected against a low-dose aerosol challenge with subspecies tularensis (strain Schu S4). These data indicate that parenterally administered non-living vaccines can be used for effective immunisation against aerosol challenges with Subspecies holarctica, although not high virulence strains of F. tularensis. Crown Copyright (C) 2007 Published by Elsevier Ltd. All rights reserved.