The effect of high frequency electric pulses on muscle contractions and antitumor efficiency in vivo for a potential use in clinical electrochemotherapy
The effect of high frequency electric pulses on muscle contractions and antitumor efficiency in vivo for a potential use in clinical electrochemotherapy
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DOI：
10.1016/j.bioelechem.2004.07.004
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发表时间：
2005-02-01
影响因子：
5
通讯作者：
Sersa, G
中科院分区：
文献类型：
--
作者：
Miklavcic, D;Pucihar, G;Sersa, G
对于接受电化学疗法的患者，肌肉收缩是不愉快感觉的主要来源。收缩是高压脉冲输送的结果。这些脉冲的相对低的重复频率（1Hz）导致与递送的每个单个脉冲相关联的单独的肌肉收缩。只要电化学疗法的抗肿瘤效果保持不变，通过将电脉冲的频率提高到强直性收缩的频率以上，就有可能减少不愉快感觉的数量。本文通过测量不同脉冲重复频率和两种不同脉冲幅度下大鼠肌肉的扭矩，以及研究不同脉冲重复频率下的电化学疗法对小鼠肿瘤的抗肿瘤效果，对上述假设进行了研究。肌肉扭矩的测量结果证实，高于强直收缩频率（> 100 Hz）的脉冲频率将单个收缩的次数减少到单个肌肉收缩。无论脉冲幅度如何，随着脉冲频率的增加，肌肉扭矩增加到100或200 Hz的频率，然后降低到与施加1 Hz脉冲串后的值类似的值。具有较高重复频率的体内电化学疗法抑制肿瘤生长，并且在所有检查的脉冲频率（1 Hz - 5 kHz）下都是有效的。这些结果表明，有一个相当大的潜力，为临床使用的高频脉冲电化学治疗。(c)2004 Elsevier B. V.保留所有权利。
Muscle contractions present the main source of unpleasant sensations for patients undergoing electrochemotherapy. The contractions are a consequence of high voltage pulse delivery. Relatively low repetition frequency of these pulses (1 Hz) results in separate muscle contractions associated with each, single pulse that is delivered. It would be possible to reduce the number of unpleasant sensations by increasing the frequency of electric pulses above the frequency of tetanic contraction, provided that the antitumor efficiency of electrochemotherapy remains the same. These assumptions were investigated in the present paper by measuring the muscle torque at different pulse repetition frequencies and at two different pulse amplitudes in rats and studying the antitumor efficiency of electrochemotherapy at different pulse repetition frequencies on tumors in mice. Measurements of muscle torque confirmed that pulse frequencies above the frequency of tetanic contraction (> 100 Hz) reduce the number of individual contractions to a single muscle contraction. Regardless of the pulse amplitude, with increasing pulse frequency muscle torque increases up to the frequency of 100 or 200 Hz and then decreases to a value similar to that after application of a 1 Hz pulse train. Electrochemotherapy in vivo with higher repetition frequencies inhibits tumor growth and is efficient at all pulse frequencies examined (1 Hz - 5 kHz). These results suggest that there is a considerable potential for clinical use of high frequency pulses in electrochemotherapy. (c) 2004 Elsevier B.V. All rights reserved.